AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 286: L1194-L1201, 2004. First published January 29, 2004; doi:10.1152/ajplung.00427.2003
1040-0605/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/6/L1194    most recent
00427.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Everett, A. D.
Right arrow Articles by Tucker, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Everett, A. D.
Right arrow Articles by Tucker, A.

Hepatoma-derived growth factor is a pulmonary endothelial cell-expressed angiogenic factor

Allen D. Everett,1,2 Jill V. Narron,1 Tamara Stoops,1 Hideji Nakamura,3 and Amy Tucker2,4

1Department of Pediatrics, Division of Pediatric Cardiology, 4Department of Internal Medicine, Cardiovascular Division, 2Cardiovascular Research Center, University of Virginia Health System, Charlottesville, Virginia 22908; and 3Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan

Submitted 3 December 2003 ; accepted in final form 27 January 2004

Hepatoma-derived growth factor (HDGF) was previously identified as a developmentally regulated cardiovascular and renal gene that is mitogenic for vascular smooth muscle and aortic endothelial cells. As reciprocal interactions of smooth muscle and endothelial cells are necessary for vascular formation, we examined whether HDGF plays a role in angiogenesis. According to immunohistochemistry, HDGF was highly expressed in endothelial cells of nonmuscularized, forming blood vessels of the fetal lung. HDGF was also expressed in endothelial cells of small (20 µm) mature arteries and veins. By Western immunoblotting, HDGF was highly expressed by human pulmonary microvascular endothelial cells in vitro. Adenoviral overexpression of HDGF was mitogenic for human pulmonary microvascular endothelial cells in serum-free medium, stimulating a 1.75-fold increase in bromodeoxyuridine (BrdU) uptake and a twofold increase in cell migration. With the chick chorioallantoic membrane (CAM), a biologic assay for angiogenesis, exogenous recombinant HDGF significantly stimulated blood vessel formation and a dose-dependent reorganization of cells within the CAM into a more compact, linear alignment reminiscent of tube formation. According to double immunostaining for endothelial cells with a transforming growth factor-{beta}II receptor antibody and BrdU as a marker of cell proliferation, exogenous HDGF selectively stimulated endothelial cell BrdU uptake. HDGF also activated specific ERK1/2 signaling and did not overlap with VEGF SAPK/JNK, Akt-mediated pathways. We conclude that HDGF is a highly expressed vascular endothelial cell protein in vivo and is a potent endothelial mitogen and regulator of endothelial cell migration by mechanisms distinct from VEGF.

lung; angiogenesis; growth factors; cell migration


Address for reprint requests and other correspondence: A. D. Everett, Johns Hopkins Hospital, Pediatric Cardiology, Brady 5, 600 N. Wolfe St., Baltimore, MD 21287 (E-mail: aeveret3{at}jhmi.edu).




This article has been cited by other articles:


Home page
 Ann. Surg. Oncol.Home page
S. Yamamoto, Y. Tomita, Y. Hoshida, E. Morii, T. Yasuda, Y. Doki, K. Aozasa, H. Uyama, H. Nakamura, and M. Monden
Expression Level of Hepatoma-Derived Growth Factor Correlates with Tumor Recurrence of Esophageal Carcinoma
Ann. Surg. Oncol., July 1, 2007; 14(7): 2141 - 2149.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
H. Uyama, Y. Tomita, H. Nakamura, S. Nakamori, B. Zhang, Y. Hoshida, H. Enomoto, Y. Okuda, M. Sakon, K. Aozasa, et al.
Hepatoma-Derived Growth Factor Is a Novel Prognostic Factor for Patients with Pancreatic Cancer.
Clin. Cancer Res., October 15, 2006; 12(20): 6043 - 6048.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
S. M. Lukasik, T. Cierpicki, M. Borloz, J. Grembecka, A. Everett, and J. H. Bushweller
High resolution structure of the HDGF PWWP domain: A potential DNA binding domain
Protein Sci., February 1, 2006; 15(2): 314 - 323.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Zhang, H. Ren, P. Yuan, W. Lang, L. Zhang, and L. Mao
Down-regulation of Hepatoma-Derived Growth Factor Inhibits Anchorage-Independent Growth and Invasion of Non-Small Cell Lung Cancer Cells
Cancer Res., January 1, 2006; 66(1): 18 - 23.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. M. Abouzied, H. M. El-tahir, L. Prenner, H. Haberlein, V. Gieselmann, and S. Franken
Hepatoma-derived Growth Factor: SIGNIFICANCE OF AMINO ACID RESIDUES 81-100 IN CELL SURFACE INTERACTION AND PROLIFERATIVE ACTIVITY
J. Biol. Chem., March 25, 2005; 280(12): 10945 - 10954.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.