AJP - Lung Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 287: L1-L23, 2004; doi:10.1152/ajplung.00301.2003
1040-0605/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (38)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hirsch, J.
Right arrow Articles by Matthay, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hirsch, J.
Right arrow Articles by Matthay, M. A.

Invited Review

Proteomics: current techniques and potential applications to lung disease

Jan Hirsch,1 Kirk C. Hansen,2 Alma L. Burlingame,2 and Michael A. Matthay1

1Cardiovascular Research Institute and 2Mass Spectrometry Facility, Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143

Proteomics aims to study the whole protein content of a biological sample in one set of experiments. Such an approach has the potential value to acquire an understanding of the complex responses of an organism to a stimulus. The large vascular and air space surface area of the lung expose it to a multitude of stimuli that can trigger a variety of responses by many different cell types. This complexity makes the lung a promising, but also challenging, target for proteomics. Important steps made in the last decade have increased the potential value of the results of proteomics studies for the clinical scientist. Advances in protein separation and staining techniques have improved protein identification to include the least abundant proteins. The evolution in mass spectrometry has led to the identification of a large part of the proteins of interest rather than just describing changes in patterns of protein spots. Protein profiling techniques allow the rapid comparison of complex samples and the direct investigation of tissue specimens. In addition, proteomics has been complemented by the analysis of posttranslational modifications and techniques for the quantitative comparison of different proteomes. These methodologies have made the application of proteomics on the study of specific diseases or biological processes under clinically relevant conditions possible. The quantity of data that is acquired with these new techniques places new challenges on data processing and analysis. This article provides a brief review of the most promising proteomics methods and some of their applications to pulmonary research.

mass spectrometry; proteome; lung


Address for reprint requests and other correspondence: J. Hirsch, Cardiovascular Research Institute, Univ. of California, San Francisco, 505 Parnassus Ave. HSW 825, San Francisco, CA 94143-0130 (E-mail: jhirsch{at}itsa.ucsf.edu).




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. B. Ware and M. A. Matthay
Beyond fishing: the role of discovery proteomics in mechanistic lung research
Am J Physiol Lung Cell Mol Physiol, January 1, 2009; 296(1): L12 - L13.
[Full Text] [PDF]

Home page
 PhysiologyHome page
T. Pisitkun, J. D. Hoffert, M.-J. Yu, and M. A. Knepper
Tandem Mass Spectrometry in Physiology
Physiology, December 1, 2007; 22(6): 390 - 400.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Santos, R. S. Moura, S. Gonzaga, C. Nogueira-Silva, S. Ohlmeier, and J. Correia-Pinto
Embryonic Essential Myosin Light Chain Regulates Fetal Lung Development in Rats
Am. J. Respir. Cell Mol. Biol., September 1, 2007; 37(3): 330 - 338.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. Hirsch, K. C. Hansen, A. Sapru, J. A. Frank, R. J. Chalkley, X. Fang, J. C. Trinidad, P. Baker, A. L. Burlingame, and M. A. Matthay
Impact of Low and High Tidal Volumes on the Rat Alveolar Epithelial Type II Cell Proteome
Am. J. Respir. Crit. Care Med., May 15, 2007; 175(10): 1006 - 1013.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
E. S. Chen and D. R. Moller
Expression Profiling in Granulomatous Lung Disease
Proceedings of the ATS, January 1, 2007; 4(1): 101 - 107.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
R. P. Bowler, M. C. Ellison, and N. Reisdorph
Proteomics in pulmonary medicine.
Chest, August 1, 2006; 130(2): 567 - 574.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
E. Kriegova, C. Melle, V. Kolek, B. Hutyrova, F. Mrazek, A. Bleul, R. M. du Bois, F. von Eggeling, and M. Petrek
Protein Profiles of Bronchoalveolar Lavage Fluid from Patients with Pulmonary Sarcoidosis
Am. J. Respir. Crit. Care Med., May 15, 2006; 173(10): 1145 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. M. Wu, M. Jin, and C. B. Marsh
Toward functional proteomics of alveolar macrophages
Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L585 - L595.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. A. Granville and P. A. Dennis
An Overview of Lung Cancer Genomics and Proteomics
Am. J. Respir. Cell Mol. Biol., March 1, 2005; 32(3): 169 - 176.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.