| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229; and 2Department of Pediatrics, University of Utah, Salt Lake City, Utah 34132
Submitted 8 January 2004 ; accepted in final form 14 March 2004
To determine whether increased levels of VEGF disrupt postnatal lung formation or function, conditional transgenic mice in which VEGF 164 expression was enhanced in respiratory epithelial cells were produced. VEGF expression was induced in the lungs of VEGF transgenic pups with doxycycline from postnatal day 1 through 2 and 6 wk of age. VEGF levels were higher in bronchoalveolar lavage fluid (BALF) and lung homogenates of VEGF transgenic mice compared with endogenous VEGF levels in controls. Neonatal mortality was increased by 50% in VEGF transgenic mice. Total protein content in BALF was elevated in VEGF transgenic mice. Surfactant protein B protein expression was unaltered in VEGF transgenic mice. Although postnatal alveolar and vascular development were not disrupted by VEGF expression, VEGF transgenic mice developed pulmonary hemorrhage, alveolar remodeling, and macrophage accumulation as early as 2 wk of age. Electron microscopy demonstrated abnormal alveolar capillary endothelium in the VEGF transgenic mice. In many locations, the endothelium was discontinuous with segments of attenuated endothelial cells. Large numbers of hemosiderin-laden macrophages and varying degrees of emphysema were observed in adult VEGF transgenic mice. Overexpression of VEGF in the neonatal lung increased infant mortality and caused pulmonary hemorrhage, hemosiderosis, alveolar remodeling, and inflammation.
chronic lung disease of infancy; bronchopulmonary dysplasia; respiratory distress syndrome; sudden infant death syndrome; emphysema; surfactant protein B
This article has been cited by other articles:
|
|
 |
|
  V. Bhandari, R. Choo-Wing, C. G. Lee, K. Yusuf, J. H. Nedrelow, N. Ambalavanan, H. Malkus, R. J. Homer, and J. A. Elias Developmental Regulation of NO-Mediated VEGF-Induced Effects in the Lung Am. J. Respir. Cell Mol. Biol., October 1, 2008; 39(4): 420 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ahlbrecht, J. Schmitz, U. Seay, C. Schwarz, R. Mittnacht-Kraus, A. Gaumann, R. V. Haberberger, S. Herold, G. Breier, F. Grimminger, et al. Spatiotemporal Expression of flk-1 in Pulmonary Epithelial Cells during Lung Development Am. J. Respir. Cell Mol. Biol., August 1, 2008; 39(2): 163 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Irwin, K. Helm, N. Campbell, M. Imamura, K. Fagan, J. Harral, M. Carr, K. A. Young, D. Klemm, S. Gebb, et al. Neonatal lung side population cells demonstrate endothelial potential and are altered in response to hyperoxia-induced lung simplification Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L941 - L951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Krenn, W. Klepetko, S. Taghavi, P. Paulus, and S. Aharinejad Vascular endothelial growth factor increases pulmonary vascular permeability in cystic fibrosis patients undergoing lung transplantation Eur. J. Cardiothorac. Surg., July 1, 2007; 32(1): 35 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Kunig, V. Balasubramaniam, N. E. Markham, G. Seedorf, J. Gien, and S. H. Abman Recombinant human VEGF treatment transiently increases lung edema but enhances lung structure after neonatal hyperoxia Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L1068 - L1078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Mehta and A Petrova Intrauterine neutrophil activation is associated with pulmonary haemorrhage in preterm infants Arch. Dis. Child. Fetal Neonatal Ed., November 1, 2006; 91(6): F415 - F418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. Meyerholz, B. Grubor, T. Lazic, J. M. Gallup, M. M. A. de Macedo, P. B. McCray, and M. R. Ackermann Monocytic/Macrophagic pneumonitis after intrabronchial deposition of vascular endothelial growth factor in neonatal lambs. Vet. Pathol., September 1, 2006; 43(5): 689 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A R L Medford and A B Millar Vascular endothelial growth factor (VEGF) in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS): paradox or paradigm? Thorax, July 1, 2006; 61(7): 621 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. F. Voelkel, R. W. Vandivier, and R. M. Tuder Vascular endothelial growth factor in the lung Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L209 - L221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. C. Carpenter, S. Schomberg, and K. R. Stenmark Endothelin-mediated increases in lung VEGF content promote vascular leak in young rats exposed to viral infection and hypoxia Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1075 - L1082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Stenmark and V. Balasubramaniam Angiogenic Therapy for Bronchopulmonary Dysplasia: Rationale and Promise Circulation, October 18, 2005; 112(16): 2383 - 2385. [Full Text] [PDF]
|
|
|
|
|
|
A. M. Kunig, V. Balasubramaniam, N. E. Markham, D. Morgan, G. Montgomery, T. R. Grover, and S. H. Abman Recombinant human VEGF treatment enhances alveolarization after hyperoxic lung injury in neonatal rats Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L529 - L535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Becker, J. Waltenberger, R. Yachechko, T. Mirzapoiazova, J. S.K. Sham, C. G. Lee, J. A. Elias, and A. D. Verin Neuropilin-1 Regulates Vascular Endothelial Growth Factor-Mediated Endothelial Permeability Circ. Res., June 24, 2005; 96(12): 1257 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. McGrath-Morrow, C. Cho, C. Cho, L. Zhen, D. J. Hicklin, and R. M. Tuder Vascular Endothelial Growth Factor Receptor 2 Blockade Disrupts Postnatal Lung Development Am. J. Respir. Cell Mol. Biol., May 1, 2005; 32(5): 420 - 427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Belteki, J. Haigh, N. Kabacs, K. Haigh, K. Sison, F. Costantini, J. Whitsett, S. E. Quaggin, and A. Nagy Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction Nucleic Acids Res., March 22, 2005; 33(5): e51 - e51. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
