| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
TRANSLATIONAL PHYSIOLOGY
Department of Anaesthetics and Intensive Care, Faculty of Medicine, Imperial College London, Chelsea and Westminster Hospital, London SW10 9NH, United Kingdom
Submitted 19 May 2004 ; accepted in final form 14 July 2004
Polymorphonuclear leukocytes (PMN) play an important role in ventilator-induced lung injury (VILI), but the mechanisms of pulmonary PMN recruitment, particularly early intravascular PMN sequestration during VILI, have not been elucidated. We investigated the physiological and molecular mechanisms of pulmonary PMN sequestration in an in vivo mouse model of VILI. Anesthetized C57/BL6 mice were ventilated for 1 h with high tidal volume (injurious ventilation), low tidal volume and high positive end-expiratory pressure (protective ventilation), or normal tidal volume (control ventilation). Pulmonary PMN sequestration analyzed by flow cytometry of lung cell suspensions was substantially enhanced in injurious ventilation compared with protective and control ventilation, preceding development of physiological signs of lung injury. Anesthetized, spontaneously breathing mice with continuous positive airway pressure demonstrated that raised alveolar pressure alone does not induce PMN entrapment. In vitro leukocyte deformability assay indicated stiffening of circulating leukocytes in injurious ventilation compared with control ventilation. PMN sequestration in injurious ventilation was markedly inhibited by administration of anti-L-selectin antibody, but not by anti-CD18 antibody. These results suggest that mechanical ventilatory stress initiates pulmonary PMN sequestration early in the course of VILI, and this phenomenon is associated with stretch-induced inflammatory events leading to PMN stiffening and mediated by L-selectin-dependent but CD18-independent mechanisms.
leukocytes; lung inflammation; mechanical ventilation
This article has been cited by other articles:
|
|
 |
|
  N. Soni and P. Williams Positive pressure ventilation: what is the real cost? Br. J. Anaesth., October 1, 2008; 101(4): 446 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Eckle, L. Fullbier, A. Grenz, and H. K. Eltzschig Usefulness of pressure-controlled ventilation at high inspiratory pressures to induce acute lung injury in mice Am J Physiol Lung Cell Mol Physiol, October 1, 2008; 295(4): L718 - L724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J A Frank, J-F Pittet, C Wray, and M A Matthay Protection from experimental ventilator-induced acute lung injury by IL-1 receptor blockade Thorax, February 1, 2008; 63(2): 147 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Oeckler and R. D. Hubmayr Ventilator-associated lung injury: a search for better therapeutic targets Eur. Respir. J., December 1, 2007; 30(6): 1216 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Thammanomai, A. Majumdar, E. Bartolak-Suki, and B. Suki Effects of reduced tidal volume ventilation on pulmonary function in mice before and after acute lung injury J Appl Physiol, November 1, 2007; 103(5): 1551 - 1559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. H. Hillman, T. J. M. Moss, S. G. Kallapur, C. Bachurski, J. J. Pillow, G. R. Polglase, I. Nitsos, B. W. Kramer, and A. H. Jobe Brief, Large Tidal Volume Ventilation Initiates Lung Injury and a Systemic Response in Fetal Sheep Am. J. Respir. Crit. Care Med., September 15, 2007; 176(6): 575 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Wilson, M. E. Goddard, K. P. O'Dea, S. Choudhury, and M. Takata Differential roles of p55 and p75 tumor necrosis factor receptors on stretch-induced pulmonary edema in mice Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L60 - L68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Wagner and J. Jenkins Effects of airway distension on leukocyte recruitment in the mouse tracheal microvasculature J Appl Physiol, April 1, 2007; 102(4): 1528 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Jacono, Y.-J. Peng, D. Nethery, J. A. Faress, Z. Lee, J. A. Kern, and N. R. Prabhakar Acute lung injury augments hypoxic ventilatory response in the absence of systemic hypoxemia J Appl Physiol, December 1, 2006; 101(6): 1795 - 1802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Allen, B. T. Suratt, L. Rinaldi, J. M. Petty, and J. H. T. Bates Choosing the frequency of deep inflation in mice: balancing recruitment against ventilator-induced lung injury Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L710 - L717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Miyao, Y. Suzuki, K. Takeshita, H. Kudo, M. Ishii, R. Hiraoka, K. Nishio, T. Tamatani, S. Sakamoto, M. Suematsu, et al. Various adhesion molecules impair microvascular leukocyte kinetics in ventilator-induced lung injury Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1059 - L1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ikegami, J. A. Whitsett, P. C. Martis, and T. E. Weaver Reversibility of lung inflammation caused by SP-B deficiency Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L962 - L970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. O'Dea, A. J. Young, H. Yamamoto, J. L. Robotham, F. M. Brennan, and M. Takata Lung-marginated Monocytes Modulate Pulmonary Microvascular Injury during Early Endotoxemia Am. J. Respir. Crit. Care Med., November 1, 2005; 172(9): 1119 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Chapman, S. E. Sinclair, D. Zhuang, A. Hassid, L. P. Desai, and C. M. Waters Cyclic mechanical strain increases reactive oxygen species production in pulmonary epithelial cells Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L834 - L841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ghosh, M. R. Wilson, S. Choudhury, H. Yamamoto, M. E. Goddard, B. Falusi, N. Marczin, and M. Takata Effects of inhaled carbon monoxide on acute lung injury in mice Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1003 - L1009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. N. Tremblay and A. S. Slutsky Pathogenesis of ventilator-induced lung injury: trials and tribulations Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L596 - L598. [Full Text] [PDF]
|
|
|
|
|
|
M. R. Wilson, S. Choudhury, and M. Takata Pulmonary inflammation induced by high-stretch ventilation is mediated by tumor necrosis factor signaling in mice Am J Physiol Lung Cell Mol Physiol, April 1, 2005; 288(4): L599 - L607. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
