AJP - Lung AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 287: L1186-L1192, 2004. First published August 20, 2004; doi:10.1152/ajplung.00170.2004
1040-0605/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
287/6/L1186    most recent
00170.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cavarra, E.
Right arrow Articles by Lungarella, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cavarra, E.
Right arrow Articles by Lungarella, G.

Early response to bleomycin is characterized by different cytokine and cytokine receptor profiles in lungs

Eleonora Cavarra,1 Fabio Carraro,2 Silvia Fineschi,1 Antonella Naldini,2 Barbara Bartalesi,1 Annalisa Pucci,2 and Giuseppe Lungarella1

Departments of 1Physiopathology, Experimental Medicine & Public Health and 2Physiology, University of Siena, Siena, Italy I-53100

Submitted 12 May 2004 ; accepted in final form 18 August 2004

The sensitivity to the fibrosis-inducing effect of bleomycin varies considerably from species to species, the reasons for which are unknown. The variability of the response in different strains of mice is well documented. Recent evidence indicates that the upregulated expression of cytokines and cytokine receptors may be involved. We evaluated the expression pattern of some cytokines and their receptors in C57Bl/6J bleomycin-sensitive and Balb/C bleomycin-resistant mice. Animals from both strains received, under ether anesthesia, either saline (50 µl) or bleomycin (0.1 U/50 µl) intratracheally. At various times after the treatment, the lungs were analyzed for cytokines and cytokine receptors by histochemistry and their mRNA by RNase protection assay. A significantly increased expression of TNF-{alpha} and IL-1{beta} was observed in both strains. However, an upregulated lung expression for TNF-{alpha} and IL-1 receptors was observed in C57Bl/6J-sensitive animals only. This profile is evident from 63 h onward. In addition to TNF-{alpha}, bleomycin administration also resulted in the upregulated expression of TGF-{beta} in the lungs of both strains at 8 h and in an enhanced expression of TGF-{beta} receptors I and II in C57Bl/6J mice only. The upregulation of TGF-{beta} receptor expression was preceded in this strain by an increased expression of IL-4, IL-13, and IL-13 receptor-{alpha} (at 8 h after bleomycin) and followed by an upregulation of gp130 and IL-6. The difference we observed in the cytokine receptor profile may offer an additional explanation for the different fibrogenic response of the two mouse strains to bleomycin.

mouse strains; early fibrogenic response; cytokine and receptor profile


Address for reprint requests and other correspondence: G. Lungarella, Dept. of Physiopathology, Experimental Medicine & Public Health, Univ. of Siena, Via Aldo Moro, Siena, Italy I-53100 (E-mail: lungarella{at}unisi.it)




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. Matute-Bello, C. W. Frevert, and T. R. Martin
Animal models of acute lung injury
Am J Physiol Lung Cell Mol Physiol, September 1, 2008; 295(3): L379 - L399.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
F. Saito, S. Tasaka, K.-i. Inoue, K. Miyamoto, Y. Nakano, Y. Ogawa, W. Yamada, Y. Shiraishi, N. Hasegawa, S. Fujishima, et al.
Role of Interleukin-6 in Bleomycin-Induced Lung Inflammatory Changes in Mice
Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 566 - 571.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
T J Williams and J W Wilson
Challenges in pulmonary fibrosis: 7 {middle dot} Novel therapies and lung transplantation
Thorax, March 1, 2008; 63(3): 277 - 284.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. Pottier, C. Chupin, V. Defamie, B. Cardinaud, R. Sutherland, G. Rios, F. Gauthier, P. J. Wolters, Y. Berthiaume, P. Barbry, et al.
Relationships between Early Inflammatory Response to Bleomycin and Sensitivity to Lung Fibrosis: A Role for Dipeptidyl-Peptidase I and Tissue Inhibitor of Metalloproteinase-3?
Am. J. Respir. Crit. Care Med., December 1, 2007; 176(11): 1098 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. R. Young, R. Pasula, P. M. Gulleman, G. H. Deutsch, and F. X. McCormack
Susceptibility of Hermansky-Pudlak Mice to Bleomycin-Induced Type II Cell Apoptosis and Fibrosis
Am. J. Respir. Cell Mol. Biol., July 1, 2007; 37(1): 67 - 74.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. Golan-Gerstl, S. B. Wallach-Dayan, G. Amir, and R. Breuer
Epithelial Cell Apoptosis by Fas Ligand-Positive Myofibroblasts in Lung Fibrosis
Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 270 - 275.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F. J. Jacono, Y.-J. Peng, D. Nethery, J. A. Faress, Z. Lee, J. A. Kern, and N. R. Prabhakar
Acute lung injury augments hypoxic ventilatory response in the absence of systemic hypoxemia
J Appl Physiol, December 1, 2006; 101(6): 1795 - 1802.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A.-M. Lemay and C. K. Haston
Bleomycin-induced pulmonary fibrosis susceptibility genes in AcB/BcA recombinant congenic mice
Physiol Genomics, September 21, 2005; 23(1): 54 - 61.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.