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This Article Full Text Full Text (PDF) All Versions of this Article: 288/1/L125    most recent 00244.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Moreno, L. Articles by Perez-Vizcaino, F. Search for Related Content PubMed PubMed Citation Articles by Moreno, L. Articles by Perez-Vizcaino, F.

Soluble guanylyl cyclase during postnatal porcine pulmonary maturation

¹Department of Pharmacology, Institute of Pharmacology and Toxicology, School of Medicine, Universidad Complutense and ³Instituto de Cardiología, Hospital Universitario San Carlos, Madrid, Spain; and ²Department of Pediatrics, University Hospital Maastricht, Research Institute Growth and Development, Maastricht, The Netherlands

Submitted 29 June 2004 ; accepted in final form 15 September 2004

The nitric oxide (NO)/cGMP pathway plays a key role in the regulation of pulmonary vascular tone during the transition from the fetal to the neonatal circulation, and it is impaired in pathophysiological conditions such as pulmonary hypertension. In the present study, we have analyzed the changes in the function and expression of soluble guanylyl cyclase (sGC) in pulmonary arteries during early postnatal maturation in isolated third-branch pulmonary arteries from newborn (3–18 h of age) and 2-wk-old piglets. The expression of sGC ₁-subunit in pulmonary arteries increased with postnatal age both at the level of mRNA and protein. The catalytic region of porcine sGC ₁ was sequenced, showing a 92% homology with the human sequence. This age-dependent increase in sGC expression correlated with increased vasorelaxant responses to the physiological sGC activator NO and to the exogenous sGC activator YC-1, but not to the membrane-permeable cGMP analog 8-bromoguanosine 3',5'-cyclic monophosphate. In conclusion, an increased expression of sGC in pulmonary conduit arteries from 2-wk-old compared with newborn piglets explains, at least partly, the age-dependent increase in the vasorelaxant response of NO and other activators of sGC.

newborn; YC-1; vascular smooth muscle

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