AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 288: L546-L551, 2005. First published November 12, 2004; doi:10.1152/ajplung.00107.2004
1040-0605/05 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Movie
Right arrow All Versions of this Article:
288/3/L546    most recent
00107.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wyatt, T. A.
Right arrow Articles by Sisson, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wyatt, T. A.
Right arrow Articles by Sisson, J. H.

Both cAMP and cGMP are required for maximal ciliary beat stimulation in a cell-free model of bovine ciliary axonemes

Todd A. Wyatt,1,2 Mary A. Forgèt,2 Jennifer M. Adams,2 and Joseph H. Sisson2

1Research Service, Department of Veterans Affairs Medical Center; and 2Pulmonary and Critical Care Medicine Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska

Submitted 24 March 2004 ; accepted in final form 8 November 2004

Previously, we have shown that the ATPase-dependent motion of cilia in bovine bronchial epithelial cells (BBEC) can be regulated through the cyclic nucleotides, cAMP via the cAMP-dependent protein kinase (PKA) and cGMP via the cGMP-dependent protein kinase (PKG). Both cyclic nucleotides cause an increase in cilia beat frequency (CBF). We hypothesized that cAMP and cGMP may act directly at the level of the ciliary axoneme in BBEC. To examine this, we employed a novel cell-free system utilizing detergent-extracted axonemes. Axoneme movement was whole-field analyzed digitally with the Sisson-Ammons video analysis system. A suspension of extracted axonemes remains motionless until the addition of 1 mM ATP that establishes a baseline CBF similar to that seen when analyzing intact ciliated BBEC. Adding 10 µM cAMP or 10 µM cGMP increases CBF beyond the established ATP baseline. However, the cyclic nucleotides did not stimulate CBF in the absence of ATP. Therefore, the combination of cAMP and cGMP augments ATP-driven CBF increases at the level of isolated axoneme.

cilia; adenosine 3',5'-cyclic monophosphate; guanosine 3',5'-cyclic monophosphate


Address for reprint requests and other correspondence: T. A. Wyatt, Dept. of Internal Medicine, Pulmonary and Critical Care Medicine Sect., Univ. of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198-5300 (E-mail: twyatt{at}unmc.edu)




This article has been cited by other articles:


Home page
 Eur Respir JHome page
T. A. Wyatt, J. H. Sisson, S. G. Von Essen, J. A. Poole, and D. J. Romberger
Exposure to hog barn dust alters airway epithelial ciliary beating
Eur. Respir. J., June 1, 2008; 31(6): 1249 - 1255.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
R. E. Slager, J. M. DeVasure, J. A. Pavlik, J. H. Sisson, and T. A. Wyatt
RACK1, a PKC Targeting Protein, Is Exclusively Localized to Basal Airway Epithelial Cells
J. Histochem. Cytochem., January 1, 2008; 56(1): 7 - 14.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
A. Schmid, Z. Sutto, M.-C. Nlend, G. Horvath, N. Schmid, J. Buck, L. R. Levin, G. E. Conner, N. Fregien, and M. Salathe
Soluble Adenylyl Cyclase Is Localized to Cilia and Contributes to Ciliary Beat Frequency Regulation via Production of cAMP
J. Gen. Physiol., July 1, 2007; 130(1): 99 - 109.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. L. Stout, T. A. Wyatt, J. J. Adams, and J. H. Sisson
Nitric Oxide-dependent Cilia Regulatory Enzyme Localization in Bovine Bronchial Epithelial Cells
J. Histochem. Cytochem., May 1, 2007; 55(5): 433 - 442.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. K. Elliott, J. H. Sisson, and T. A. Wyatt
Effects of Cigarette Smoke and Alcohol on Ciliated Tracheal Epithelium and Inflammatory Cell Recruitment
Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 452 - 459.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Schmid, G. Bai, N. Schmid, M. Zaccolo, L. E. Ostrowski, G. E. Conner, N. Fregien, and M. Salathe
Real-time analysis of cAMP-mediated regulation of ciliary motility in single primary human airway epithelial cells
J. Cell Sci., October 15, 2006; 119(20): 4176 - 4186.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. Delmotte and M. J. Sanderson
Ciliary Beat Frequency Is Maintained at a Maximal Rate in the Small Airways of Mouse Lung Slices
Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 110 - 117.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Vent, T. A. Wyatt, D. D. Smith, A. Banerjee, R. F. Luduena, J. H. Sisson, and R. Hallworth
Direct involvement of the isotype-specific C-terminus of {beta} tubulin in ciliary beating
J. Cell Sci., October 1, 2005; 118(19): 4333 - 4341.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.