| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Department of Pharmacy, 2Dorothy M. Davis Heart and Lung Research Institute, and 3Division of Pulmonary, Critical Care, and Sleep Medicine, The Ohio State University, Columbus, Ohio
Submitted 4 August 2005 ; accepted in final form 20 September 2005
The accelerated loss of lung epithelium through activation of extrinsic apoptosis is believed to play a causative role in lung pathogenesis. Previous investigations have shown that zinc is required to sustain lung epithelial cell viability under stress conditions and that depletion of intracellular zinc predisposes cells to apoptosis. In this investigation, we determined whether intracellular zinc deficiency enhanced the susceptibility of primary, differentiated cultures of human lung epithelium to death receptor-mediated apoptosis, leading to barrier dysfunction. Cultures obtained from multiple donors were exposed to stimuli that provoke death receptor-mediated apoptosis and depleted of intracellular zinc with a zinc-specific chelating agent. Transepithelial resistance, paracellular transport, caspase-8 and caspase-3 activity, and apoptosis were measured. Activation of extrinsic apoptosis or zinc chelation alone resulted in a nominal increase in caspase function and apoptosis without major evidence of barrier disruption. Activation of extrinsic apoptosis in addition to zinc depletion resulted in an abrupt decrease in transepithelial resistance, a substantial increase in apoptosis, and an increased paracellular leak. Cultures were rescued by supplementation with zinc sulfate. Further analysis revealed that exogenous zinc facilitates cell survival through activation of the phosphatidylinositol 3-kinase/Akt signaling pathway. We conclude that intracellular zinc is a vital factor in lung epithelium that protects cells from death receptor-mediated apoptosis and barrier dysfunction.
programmed cell death; airway epithelium; caspase; barrier dysfunction
This article has been cited by other articles:
|
|
 |
|
  S. Dailianis, E. Patetsini, and M. Kaloyianni The role of signalling molecules on actin glutathionylation and protein carbonylation induced by cadmium in haemocytes of mussel Mytilus galloprovincialis (Lmk) J. Exp. Biol., November 15, 2009; 212(22): 3612 - 3620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-T. Han, N. W. Schoene, and K. Y. Lei Influence of zinc deficiency on Akt-Mdm2-p53 and Akt-p21 signaling axes in normal and malignant human prostate cells Am J Physiol Cell Physiol, November 1, 2009; 297(5): C1188 - C1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Chanoit, S. Lee, J. Xi, M. Zhu, R. A. McIntosh, R. A. Mueller, E. A. Norfleet, and Z. Xu Exogenous zinc protects cardiac cells from reperfusion injury by targeting mitochondrial permeability transition pore through inactivation of glycogen synthase kinase-3{beta} Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H1227 - H1233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Besecker, S. Bao, B. Bohacova, A. Papp, W. Sadee, and D. L. Knoell The human zinc transporter SLC39A8 (Zip8) is critical in zinc-mediated cytoprotection in lung epithelia Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1127 - L1136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zhou, J. Liu, Z. Song, C. J. McClain, and Y. J. Kang Zinc Supplementation Inhibits Hepatic Apoptosis in Mice Subjected to a Long-Term Ethanol Exposure Experimental Biology and Medicine, May 1, 2008; 233(5): 540 - 548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bao and D. L. Knoell Zinc modulates cytokine-induced lung epithelial cell barrier permeability Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1132 - L1141. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
