HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/4/L828    most recent 00120.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Allahverdian, S. Articles by Dorscheid, D. R. Search for Related Content PubMed PubMed Citation Articles by Allahverdian, S. Articles by Dorscheid, D. R.

Airway epithelial wound repair: role of carbohydrate sialyl Lewis^x

¹The James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, Faculty of Medicine, St. Paul's Hospital, University of British Columbia, Vancouver, British Columbia, Canada; and ²Section of Pulmonary and Critical Care Medicine, Department of Medicine, Division of Biological Sciences, University of Chicago, Chicago, Illinois

Submitted 1 April 2006 ; accepted in final form 26 May 2006

Epithelial repair is a complex cellular and molecular process, the details of which are still not clearly understood. Plasma membrane glycoconjugates can modulate cell function by altering the function of protein and lipids. Sialyl Lewis^x (sLe^x), a fucose-containing tetrasaccharide, decorates membrane-bound and secreted proteins and mediates cell-cell interaction. In the present study we investigated the role of sLe^x in airway epithelial repair. Using immunohistochemistry, we showed an increased expression of sLe^x in areas of damaged bronchial epithelium compared with intact regions. Confluent monolayers of airway epithelial cells were mechanically wounded and allowed to close. Wounded monolayers were photographed for wound closure kinetics, fixed for immunocytochemical studies, or subjected to RNA extraction. Examining the expression of different 1,3-fucosyltransferases (FucT), enzymes that mediate the final step in the synthesis of sLe^x, we found that FucT-IV was the common gene expressed in all cell lines and primary airway epithelial cells. We demonstrated an increased expression of sLe^x over time after mechanical injury. Blocking of sLe^x with an inhibitory antibody completely prevented epithelial repair. Our data suggest an essential functional role for sLe^x in epithelial repair. Further studies are necessary to explore the exact mechanism for sLe^x in mediating cell-cell interaction in bronchial epithelial cells to facilitate epithelial migration and repair.

fucosyltransferase; selectin; airway epithelium; epidermal growth factor

This article has been cited by other articles:

				S. Allahverdian, N. Harada, G. K. Singhera, D. A. Knight, and D. R. Dorscheid Secretion of IL-13 by Airway Epithelial Cells Enhances Epithelial Repair via HB-EGF Am. J. Respir. Cell Mol. Biol., February 1, 2008; 38(2): 153 - 160. [Abstract] [Full Text] [PDF]

				B. J. Patchell, K. R. Wojcik, T.-L. Yang, S. R. White, and D. R. Dorscheid Glycosylation and annexin II cell surface translocation mediate airway epithelial wound repair Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L354 - L363. [Abstract] [Full Text] [PDF]

				E. C. Adam, S. T. Holgate, and P. M. Lackie Epithelial repair is inhibited by an {alpha}1,6-fucose binding lectin Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L462 - L468. [Abstract] [Full Text] [PDF]