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1Vascular Biology Center, Medical College of Georgia, Augusta, Georgia; and 2Department of Biomedical and Pharmaceutical Sciences, The University of Montana, Missoula, Montana
Submitted 1 November 2005 ; accepted in final form 24 August 2006
Oxidative stress has been associated with multiple pathologies and disease states, including those involving the cardiovascular system. Previously, we showed that pulmonary artery endothelial cells (PAECs) undergo apoptosis after acute exposure to H2O2. However, the underlying mechanisms regulating this process remain unclear. Because of the prevalence of H2O2 in normal physiological processes and apparent loss of regulation in disease states, the purpose of this study was to develop a more complete understanding of H2O2-mediated adverse effects on endothelial cell survival. Acute exposure of PAECs to H2O2 caused a dose-dependent increase in cellular release of lactate dehydrogenase and a significant increase in production of superoxide ions, which appear to be generated within the mitochondria, as well as a significant loss of mitochondrial membrane potential and activity. Subsequent to the loss of mitochondrial membrane potential, PAECs exhibited significant caspase activation and apoptotic nuclei. We also observed a significant increase in intracellular free Zn2+ after bolus exposure to H2O2. To determine whether this increase in Zn2+ was involved in the apoptotic pathway induced by acute H2O2 exposure, we developed an adenoviral construct for overexpression of the Zn2+-binding protein metallothionein-1. Our data indicate that chelating Zn2+, either pharmacologically with N,N,N',N-tetrakis(2-pyridylmethyl)ethylene diamine or by overexpression of the Zn2+-binding protein metallothionein-1, in PAECs conferred significant protection from induction of apoptosis and cell death associated with the effects of acute H2O2 exposure. Our results show that the acute toxicity profile of H2O2 can be attributed, at least in part, to liberation of Zn2+ within PAECs. We speculate that regulation of Zn2+ levels may represent a potential therapeutic target for cardiovascular disease associated with acute oxidative stress.
metallothionein-1; apoptosis; oxidant stress; cell signaling/signal transduction
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