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Am J Physiol Lung Cell Mol Physiol 292: L367-L377, 2007. First published October 13, 2006; doi:10.1152/ajplung.00242.2006
1040-0605/07 $8.00
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INVITED REVIEW

Exploring host-pathogen interactions at the epithelial surface: application of transcriptomics in lung biology

Joost B. Vos,1 Nicole A. Datson,2 Klaus F. Rabe,1 and Pieter S. Hiemstra1

Departments of 1Pulmonology and 2Medical Pharmacology, Leiden Amsterdam Center for Drug Research, Leiden University Medical Center, Leiden, The Netherlands

The epithelial surface of the airways is the largest barrier-forming interface between the human body and the outside world. It is now well recognized that, at this strategic position, airway epithelial cells play an eminent role in host defense by recognizing and responding to microbial exposure. Conversely, inhaled microorganisms also respond to contact with epithelial cells. Our understanding of this cross talk is limited, requiring sophisticated experimental approaches to analyze these complex interactions. High-throughput technologies, such as DNA microarray analysis and serial analysis of gene expression (SAGE), have been developed to screen for gene expression levels at large scale within single experiments. Since their introduction, these hypothesis-generating technologies have been widely used in diverse areas such as oncology and brain research. Successful application of these genomics-based technologies has also revealed novel insights in host-pathogen interactions in both the host and pathogen. This review aims to provide an overview of the SAGE and microarray technology illustrated by their application in the analysis of host-pathogen interactions. In particular, the interactions between epithelial cells in the human lungs and clinically relevant microorganisms are the central focus of this review.

large-scale gene expression profiling; serial analysis of gene expression; microarray; epithelial host defense


Address for reprint requests and other correspondence: P. S. Hiemstra, Dept. of Pulmonology, Leiden Univ. Medical Center, PO Box 9600, 2300 RC Leiden, TheNetherlands (e-mail: p.s.hiemstra{at}lumc.nl)






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