AJP - Lung Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 292: L614-L624, 2007. First published September 8, 2006; doi:10.1152/ajplung.00288.2005
1040-0605/07 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
292/3/L614    most recent
00288.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Winters, S. L.
Right arrow Articles by Boucher, R. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Winters, S. L.
Right arrow Articles by Boucher, R. C.

EDITORIAL FOCUS

Mechanosensitivity of mouse tracheal ciliary beat frequency: roles for Ca2+, purinergic signaling, tonicity, and viscosity

Scot L. Winters,1,3 C. William Davis,2,3 and Richard C. Boucher1,3

Departments of 1Medicine and 2Cell and Molecular Physiology, 3Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina

Submitted 2 July 2005 ; accepted in final form 6 September 2006

Mechanosensitivity is hypothesized to participate in the regulation of ciliary beat frequency (CBF) in airway epithelia. To investigate this hypothesis, CBF in excised mouse trachea was monitored (microscopy image analysis) while varying mucosal shear (perfusate velocity and/or viscosity; planar flow). CBF increased within minutes of step increase to steady shear stress as small as 10–3 Pa and decreased within minutes of shear reduction (≤10–4 Pa). CBF response was directional, being less with cephalad vs. caudal flow, and was reduced in trachea from mutant mice lacking P2Y2 receptors, as well as by administration of the Ca2+ chelator EGTA, the Ca2+ channel inhibitor La3+, the nucleotide phosphohydrolase apyrase, the metabolically stabilized adenosine receptor agonist 5'-(N-ethylcarboxamido)adenosine, the osmotic agent mannitol, and the viscosity modifier dextran. Brief exposure to exogenous ATP, a candidate mediator, augmented CBF response, although augmentation declined with higher ATP concentration (5.0 vs. 0.1 mM) or longer ATP exposure before shear (55 vs. 20 min). Prolonged extended exposure (45 min) to the metabolically stabilized ATP analog ATP{gamma}S [adenosine 5'-(3-thiotriphosphate), 0.1 mM] inhibited CBF response to shear. Furthermore, neither ATP nor ATP{gamma}S substantially increased CBF in the relative absence of shear. With viscosity increase or shear withdrawal apyrase evoked CBF stimulation, inhibitable by the adenosine receptor antagonist 8-(p-sulfophenyl)theophylline. Thus CBF response to shear is finely tuned, directional, La3+ sensitive, likely dependent on extracellular Ca2+ and ATP, involving P2Y2 and adenosine receptor activations, influenced by shear history, tonicity, viscosity, and metabolism/exposure of ATP, and thus reflective of a complex interplay of physical and biochemical actions.

shear; ATP; osmotic; stretch activated; mucociliary


Address for reprint requests and other correspondence: S. L. Winters, UNC Cystic Fibrosis Center, 7011 Thurston-Bowles, CB# 7248, Chapel Hill, NC 27599-7248 (e-mail: scot_winters{at}med.unc.edu)




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. J. B. Francis, B. Chatterjee, N. T. Loges, H. Zentgraf, H. Omran, and C. W. Lo
Initiation and maturation of cilia-generated flow in newborn and postnatal mouse airway
Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L1067 - L1075.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
M. K. Klein, R. V. Haberberger, P. Hartmann, P. Faulhammer, K. S. Lips, B. Krain, J. Wess, W. Kummer, and P. Konig
Muscarinic receptor subtypes in cilia-driven transport and airway epithelial development
Eur. Respir. J., May 1, 2009; 33(5): 1113 - 1121.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. A. Driscoll, S. Bhalla, H. Liapis, A. Ibricevic, and S. L. Brody
Autosomal Dominant Polycystic Kidney Disease Is Associated With an Increased Prevalence of Radiographic Bronchiectasis
Chest, May 1, 2008; 133(5): 1181 - 1188.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Fernandes, I. M. Lorenzo, Y. N. Andrade, A. Garcia-Elias, S. A. Serra, J. M. Fernandez-Fernandez, and M. A. Valverde
IP3 sensitizes TRPV4 channel to the mechano- and osmotransducing messenger 5'-6'-epoxyeicosatrienoic acid
J. Cell Biol., April 3, 2008; 181(1): 143 - 155.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Nakahari
Regulation of ciliary beat frequency in airways: shear stress, ATP action, and its modulation
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L612 - L613.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.