| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Center for Lung Regeneration, Department of Environmental and Occupational Health, University of Pittsburgh, and 2University of Pittsburgh Cancer Institute, Department of Radiation Oncology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; 3First Department of Medicine, Hokkaido University School of Medicine, Kita-ku, Japan; and 4Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania
Submitted 12 March 2006 ; accepted in final form 22 November 2006
Previous analysis of lung injury and repair has provided evidence for region-specific stem cells that maintain proximal and distal epithelial compartments. However, redundant expression of lineage markers by cells at several levels of the stem cell hierarchy has complicated phenotypic and functional characterization of clonogenic airway cells. Based on the demonstration that rapid efflux of the DNA dye Hoechst 33342 can be used to prospectively purify long-term repopulating hematopoietic stem cells, we hypothesized that lung cells with similar biochemical properties would be enriched for clonogenic progenitors. We demonstrate that Hoechst-dim side population (SP) cells isolated from proximal and distal compartments of the mouse lung were relatively small and agranular, exhibited low red and green autofluorescence, and that the SP fraction was highly enriched in clonogenic cells. Quantitative RT-PCR indicated that vimentin mRNA was enriched and that epithelial markers were depleted in these preparations of SP cells. Bleomycin exposure was associated with decreased clonogenicity among alveolar SP and suggested that SP cell function was compromised under profibrotic conditions. We conclude that the SP phenotype is common to clonogenic cells at multiple airway locations and suggest that Hoechst efflux is a property of cells expressing a wound-repair phenotype.
clonogenic airway cells
This article has been cited by other articles:
|
|
 |
|
  D. M. Raiser and C. F. Kim Commentary: Sca-1 and Cells of the Lung: A matter of Different Sorts Stem Cells, March 1, 2009; 27(3): 606 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Zemke, J. C. Snyder, B. L. Brockway, J. A. Drake, S. D. Reynolds, N. Kaminski, and B. R. Stripp Molecular Staging of Epithelial Maturation Using Secretory Cell-Specific Genes as Markers Am. J. Respir. Cell Mol. Biol., March 1, 2009; 40(3): 340 - 348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. McQualter, N. Brouard, B. Williams, B. N. Baird, S. Sims-Lucas, K. Yuen, S. K. Nilsson, P. J. Simmons, and I. Bertoncello Endogenous Fibroblastic Progenitor Cells in the Adult Mouse Lung Are Highly Enriched in the Sca-1 Positive Cell Fraction Stem Cells, March 1, 2009; 27(3): 623 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-L. Hackett, F. Shaheen, A. Johnson, S. Wadsworth, D. V. Pechkovsky, D. B. Jacoby, A. Kicic, S. M. Stick, and D. A. Knight Characterization of Side Population Cells from Human Airway Epithelium Stem Cells, October 1, 2008; 26(10): 2576 - 2585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Weiss, J. K. Kolls, L. A. Ortiz, A. Panoskaltsis-Mortari, and D. J. Prockop Stem Cells and Cell Therapies in Lung Biology and Lung Diseases Proceedings of the ATS, July 15, 2008; 5(5): 637 - 667. [Full Text] [PDF]
|
|
|
|
|
|
S. D. Reynolds, A. C. Zemke, A. Giangreco, B. L. Brockway, R. M. Teisanu, J. A. Drake, T. Mariani, P. Y.P. Di, M. M. Taketo, and B. R. Stripp Conditional Stabilization of {beta}-Catenin Expands the Pool of Lung Stem Cells Stem Cells, May 1, 2008; 26(5): 1337 - 1346. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
