HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 293/1/L105    most recent 00470.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sharma, A. K. Articles by Laubach, V. E. Search for Related Content PubMed PubMed Citation Articles by Sharma, A. K. Articles by Laubach, V. E.

Proinflammatory response of alveolar epithelial cells is enhanced by alveolar macrophage-produced TNF- during pulmonary ischemia-reperfusion injury

Departments of ¹Surgery and ²Medicine, University of Virginia Health System, Charlottesville, Virginia

Submitted 7 December 2006 ; accepted in final form 4 April 2007

Pulmonary ischemia-reperfusion (IR) injury entails acute activation of alveolar macrophages followed by neutrophil sequestration. Although proinflammatory cytokines and chemokines such as TNF- and monocyte chemoattractant protein-1 (MCP-1) from macrophages are known to modulate acute IR injury, the contribution of alveolar epithelial cells to IR injury and their intercellular interactions with other cell types such as alveolar macrophages and neutrophils remain unclear. In this study, we tested the hypothesis that following IR, alveolar macrophage-produced TNF- further induces alveolar epithelial cells to produce key chemokines that could then contribute to subsequent lung injury through the recruitment of neutrophils. Cultured RAW264.7 macrophages and MLE-12 alveolar epithelial cells were subjected to acute hypoxia-reoxygenation (H/R) as an in vitro model of pulmonary IR. H/R (3 h/1 h) significantly induced KC, MCP-1, macrophage inflammatory protein-2 (MIP-2), RANTES, and IL-6 (but not TNF-) by MLE-12 cells, whereas H/R induced TNF-, MCP-1, RANTES, MIP-1, and MIP-2 (but not KC) by RAW264.7 cells. These results were confirmed using primary murine alveolar macrophages and primary alveolar type II cells. Importantly, using macrophage and epithelial coculture methods, the specific production of TNF- by H/R-exposed RAW264.7 cells significantly induced proinflammatory cytokine/chemokine expression (KC, MCP-1, MIP-2, RANTES, and IL-6) by MLE-12 cells. Collectively, these results demonstrate that alveolar type II cells, in conjunction with alveolar macrophage-produced TNF-, contribute to the initiation of acute pulmonary IR injury via a proinflammatory cascade. The release of key chemokines, such as KC and MIP-2, by activated type II cells may thus significantly contribute to neutrophil sequestration during IR injury.

cytokines; chemokines

This article has been cited by other articles:

				L. Sun, R.-F. Guo, M. W. Newstead, T. J. Standiford, D. R. Macariola, and T. P. Shanley Effect of IL-10 on Neutrophil Recruitment and Survival after Pseudomonas aeruginosa Challenge Am. J. Respir. Cell Mol. Biol., July 1, 2009; 41(1): 76 - 84. [Abstract] [Full Text] [PDF]

				Z. Yang, A. K. Sharma, J. Linden, I. L. Kron, and V. E. Laubach CD4+ T lymphocytes mediate acute pulmonary ischemia-reperfusion injury. J. Thorac. Cardiovasc. Surg., March 1, 2009; 137(3): 695 - 702. [Abstract] [Full Text] [PDF]

				Z. Yang, A. K. Sharma, M. Marshall, I. L. Kron, and V. E. Laubach NADPH Oxidase in Bone Marrow-Derived Cells Mediates Pulmonary Ischemia-Reperfusion Injury Am. J. Respir. Cell Mol. Biol., March 1, 2009; 40(3): 375 - 381. [Abstract] [Full Text] [PDF]

				J. Xu, F. Xu, and E. Barrett Metalloelastase in lungs and alveolar macrophages is modulated by extracellular substance P in mice Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L162 - L170. [Abstract] [Full Text] [PDF]

				A. Elizur, T. L. Adair-Kirk, D. G. Kelley, G. L. Griffin, D. E. deMello, and R. M. Senior Tumor Necrosis Factor-{alpha} from Macrophages Enhances LPS-Induced Clara Cell Expression of Keratinocyte-Derived Chemokine Am. J. Respir. Cell Mol. Biol., January 1, 2008; 38(1): 8 - 15. [Abstract] [Full Text] [PDF]