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This Article Full Text Full Text (PDF) All Versions of this Article: 293/3/L651    most recent 00464.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Gandhi, S. G. Articles by O'Brodovich, H. Search for Related Content PubMed PubMed Citation Articles by Gandhi, S. G. Articles by O'Brodovich, H.

Effects of cardiogenic edema fluid on ion and fluid transport in the adult lung

¹Canadian Institutes of Health Research Group in Lung Development and ²Physiology and Experimental Medicine, ³Genetics and Genome Biology of the Research Institute of the Hospital for Sick Children, and Departments of ⁴Paediatrics, ⁵Physiology, and ⁶Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada; and Departments of ⁷Physiology and ⁸Pediatrics, Emory University, Atlanta, Georgia

Submitted 29 November 2006 ; accepted in final form 1 June 2007

We have previously shown that cardiogenic pulmonary edema fluid (EF) increases Na⁺ and fluid transport by fetal distal lung epithelia (FDLE) (Rafii B, Gillie DJ, Sulowski C, Hannam V, Cheung T, Otulakowski G, Barker PM and O'Brodovich H. J Physiol 544: 537–548, 2002). We now report the effect of EF on Na⁺ and fluid transport by the adult lung. We first studied primary cultures of adult type II (ATII) epithelium and found that overnight exposure to EF increased Na⁺ transport, and this effect was mainly due to factors other than catecholamines. Plasma did not stimulate Na⁺ transport in ATII. Purification of EF demonstrated that at least some agent(s) responsible for the amiloride-insensitive component resided within the globulin fraction. ATII exposed to globulins demonstrated a conversion of amiloride-sensitive short-circuit current (I_sc) to amiloride-insensitive I_sc with no increase in total I_sc. Patch-clamp studies showed that ATII exposed to EF for 18 h had increased the number of highly selective Na⁺ channels in their apical membrane. In situ acute exposure to EF increased the open probability of Na⁺-permeant ion channels in ATII within rat lung slices. EF did increase, by amiloride-sensitive pathways, the alveolar fluid clearance from the lungs of adult rats. We conclude that cardiogenic EF increases Na⁺ transport by adult lung epithelia in primary cell culture, in situ and in vivo.

pulmonary edema fluid; alveolar type ii epithelium; sodium channels; lung fluid clearance

This article has been cited by other articles:

				H. O'Brodovich, P. Yang, S. Gandhi, and G. Otulakowski Amiloride-insensitive Na+ and fluid absorption in the mammalian distal lung Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L401 - L408. [Abstract] [Full Text] [PDF]