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EDITORIAL FOCUS

Neutrophil caveolin-1 expression contributes to mechanism of lung inflammation and injury

Departments of ¹Pharmacology and ²Anesthesiology and ³Center for Lung and Vascular Biology, University of Illinois College of Medicine, Chicago, Illinois; and ⁴Herman B. Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana

Submitted 6 July 2007 ; accepted in final form 6 November 2007

Caveolin-1 present in immune cells may be involved in regulation of the inflammatory response. Here, using caveolin-1-null (Cav-1^–/–) mice, we addressed the role of caveolin-1 in polymorphonuclear neutrophils (PMNs) in regulating PMN activation-mediated lung injury. In lungs of wild-type (Cav-1^+/+) mice perfused at constant flow with Krebs-Henseleit solution, addition of Cav-1^+/+ PMNs (4 x 10⁶ cells) into the perfusate followed by their activation with formyl-Met-Leu-Phe (fMLP, 1.0 µM) plus platelet-activating factor (1.0 nM) increased pulmonary microvessel filtration coefficient by 150% and wet-to-dry lung weight ratio by 50% as well as PMN accumulation in lungs. These responses were markedly reduced in lungs perfused with Cav-1^–/– PMNs followed by addition of the same activating agents. fMLP-stimulated adhesion of Cav-1^–/– PMNs to pulmonary microvascular endothelial cells and migration of Cav-1^–/– PMNs across endothelial monolayers were also impaired compared with Cav-1^+/+ PMNs. Cav-1^–/– PMNs showed 50–80% reduction in PMA- or fMLP-stimulated superoxide production compared with Cav-1^+/+ PMNs. In addition, Cav-1^–/– PMNs had decreased migratory activity (50%) and adhesion to fibrinogen (40%) in response to fMLP. Rac1 and Rac2 were activated in Cav-1^+/+ PMNs after stimulation of fMLP but not in Cav-1^–/– PMNs. Exogenous expression of caveolin-1 in COS-phox cells augmented the fMLP-induced Rac1 activation and superoxide production, indicating a direct role of caveolin-1 in the mechanism of superoxide production. Thus caveolin-1 expression in PMNs plays a key role in mediating PMN activation, adhesion, and transendothelial migration and in PMN activation-induced lung inflammation and vascular injury.

caveolae; permeability; edema; Rac

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