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Am J Physiol Lung Cell Mol Physiol 296: L519-L526, 2009. First published December 31, 2008; doi:10.1152/ajplung.90534.2008
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Modulation of PKC{delta} signaling alters the shear stress-mediated increases in endothelial nitric oxide synthase transcription: role of STAT3

Neetu Sud,1 Sanjiv Kumar,1 Stephen Wedgwood,2 and Stephen M. Black1

1Vascular Biology Center, Medical College of Georgia, Augusta, Georgia; and 2Department of Pediatrics, Northwestern University, Chicago, Illinois

Submitted 27 October 2008 ; accepted in final form 29 December 2008

We have previously shown that the regulation of endothelial nitric oxide synthase (eNOS) in endothelial cells isolated from fetal lamb under static conditions is positively regulated by PKC{delta}. In this study, we explore the role of PKC{delta} in regulating shear-induced upregulation of eNOS. We found that shear caused a decrease in PKC{delta} activation. Modulation of PKC{delta} before shear with a dominant negative mutant of PKC{delta} (DN PKC{delta}) or bryostatin (a known PKC{delta} activator) demonstrated that PKC{delta} inhibition potentiates the shear-mediated increases in eNOS expression and activity, while PKC{delta} activation inhibited these events. To gain insight into the mechanism by which PKC{delta} inhibits shear-induced eNOS expression, we examined activation of STAT3, a known target for PKC{delta} phosphorylation. We found that shear decreased the phosphorylation of STAT3. Further the transfection of cells with DN PKC{delta} reduced, while PKC{delta} activation enhanced, STAT3 phosphorylation in the presence of shear. Transfection of cells with a dominant negative mutant of STAT3 enhanced eNOS promoter activity and nitric oxide production in response to shear. Finally, we found that mutating the STAT3 binding site sequence within the eNOS promoter increased promoter activity in response to shear and that this was no longer inhibited by bryostatin. In conclusion, shear decreases PKC{delta} activity and, subsequently, reduces STAT3 binding to the eNOS promoter. This signaling pathway plays a previously unidentified role in the regulation of eNOS expression by shear stress.

cell signaling; phosphorylation; endothelial cell; biomechanical forces


Address for reprint requests and other correspondence: S. M. Black, Vascular Biology Center: CB-3210B, Medical College of Georgia, 1459 Laney Walker Blvd, Augusta, GA 30912 (e-mail:sblack{at}mcg.edu)






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