AJP - Lung Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol 297: L196-L203, 2009. First published May 8, 2009; doi:10.1152/ajplung.00036.2008
1040-0605/09 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
297/1/L196    most recent
00036.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Liu, C.
Right arrow Articles by Sjöberg, J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, C.
Right arrow Articles by Sjöberg, J.

15-Lipoxygenase-1 induces expression and release of chemokines in cultured human lung epithelial cells

Cheng Liu,1,2 Dawei Xu,1 Li Liu,1,3 Frida Schain,1 Åsa Brunnström,4 Magnus Björkholm,1 Hans-Erik Claesson,4,5 and Jan Sjöberg1

1Department of Medicine, Division of Hematology, Karolinska University Hospital Solna and Karolinska Institutet, Stockholm; 4Orexo, Berzelius väg 3, Solna; 5Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden; 2Department of Urology, Qilu Hospital, and 3School of Nursing, Shandong University, Jinan, People's Republic of China

Submitted 18 January 2008 ; accepted in final form 4 May 2009

15-Lipoxygenase-1 (15-LOX-1) has been proposed to be involved in various physiological and pathophysiological activities such as inflammation, atherosclerosis, cell maturation, and tumorigenesis. Asthma and chronic obstructive pulmonary disease are associated with increased expression of 15-LOX-1 in bronchial epithelial cells, but the potential functions of 15-LOX-1 in airway epithelial cells have not been well clarified. To study the function of 15-LOX-1 in bronchial epithelial cells, we ectopically expressed 15-LOX-1 in the human lung epithelial cell line A549. We found that overexpression of 15-LOX-1 in A549 cells leads to increased release of the chemokines MIP-1{alpha}, RANTES, and IP-10, and thereby to increased recruitment of immature dendritic cells, mast cells, and activated T cells. These results suggest that an increased expression and activity of 15-LOX-1 in lung epithelial cells is a proinflammatory event in the pathogenesis of asthma and other inflammatory lung disorders.

airway epithelial cells; asthma; arachidonic acid


Address for reprint requests and other correspondence: C. Liu, Hematology Laboratory, Center for Molecular Medicine, L8:00, Karolinska Univ. Hospital Solna, SE-171 76 Stockholm, Sweden (e-mail: Cheng.liu{at}ki.se)






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2009 by the American Physiological Society.