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Am J Physiol Lung Cell Mol Physiol 274: L508-L516, 1998;
1040-0605/98 $5.00
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Vol. 274, Issue 4, L508-L516, April 1998

NOTE: The fulltext of this article is not currently available online. Please refer to the pdf for the complete article.

H2O2 causes endothelial barrier dysfunction without disrupting the arginine-nitric oxide pathway

Mahesh P. Gupta, Helmut O. Steinberg, and C. Michael Hart

Department of Medicine, Indiana University and Richard L. Roudebush Veterans Affairs Medical Centers, Indianapolis, Indiana 46202

We have previously demonstrated that nitric oxide (· NO) donors attenuate and that inhibition of endogenous nitric oxide synthase (NOS) enhances hydrogen peroxide (H2O2)-mediated porcine pulmonary artery endothelial cell (PAEC) injury. The current study investigates the hypothesis that oxidant-mediated inhibition of NOS contributes to PAEC injury. PAEC barrier function, measured as the transmonolayer clearance of albumin, was significantly impaired by H2O2 (10-100 µM) in the absence of cytotoxicity. Treatment with H2O2 did not alter NOS activity, measured as the conversion of [3H]arginine to [3H]citrulline in PAEC lysates, either immediately after treatment with 0-250 µM H2O2 for 30 min or for up to 120 min after treatment with 100 µM H2O2. H2O2 had little effect on NOS activity in intact PAECs, measured as 1) the formation of [3H]citrulline in [3H]arginine-loaded PAECs, 2) PAEC guanosine 3',5'-cyclic monophosphate content, and 3) PAEC · NO release to the culture media. These results indicate that the arginine-· NO pathway remains intact after exposure to oxidant conditions sufficient to promote functional derangements of vascular endothelial cells.

oxidants; vascular endothelium; nitric oxide synthase; hydrogen peroxide


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This Article
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PubMed
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Right arrow Articles by Gupta, M. P.
Right arrow Articles by Hart, C. M.

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