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Fig. 2. Effects of hemorrhage on HMGB1 expression among pulmonary cell populations. A: immunohistochemical staining of lungs in control mice and in mice hemorrhaged (Hem) 24 h previously. In control and hemorrhaged mice, alveolar and interstitial macrophages showed positive staining for HMGB1 (white arrows, top left and bottom). In both groups, endothelial cells also stained for HMGB1 (black arrows, top right and bottom right). After hemorrhage, neutrophils positive for HMGB1 (white arrow, right bottom) were found in the lungs. B: pulmonary expressions of HMGB1 and -actin determined by Western blot analysis in control, hemorrhaged, and neutropenic-hemorrhaged mice 24 h after blood loss. The data shown are representative of 3 independent experiments. NP, neutropenia.

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