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Fig. 5. Effect of increased TRPC1 expression on current through store-operated channels. B: human dermal microvascular endothelial cells were transfected with TRPC1 cDNA. Isolated single cells were voltage clamped to a potential of –50 mV using a patch pipette containing inositol 1,4,5-trisphosphate plus dibenzohydroquinone to deplete intracellular calcium stores (endoplasmic reticulum). Current through SOCs rapidly developed as shown. La3+ (100 µM) was added where indicated to block the current.
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