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Am J Physiol Lung Cell Mol Physiol (September 28, 2001). doi:10.1152/ajplung.00027.2001
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Articles in PresS, published online ahead of print September 28, 2001
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00027.2001
Submitted on January 31, 2001
Accepted on September 12, 2001

Maintenance of surfactant protein A and D secretion by rat alveolar type II cells in vitro

Robert J Mason1*, Michelle C Lewis1, Karen E Edeen1, Kathleen McCormick-Shannon1, Larry D Nielsen1, and John M Shannon1

1 Department of Medicine, National Jewish, Denver, CO, USA

* To whom correspondence should be addressed. E-mail: masonb{at}njc.org.

Secretion of surfactant proteins A and D (SP-A and SP-D) has been difficult to study in vitro because a culture system for maintaining surfactant secretion has been difficult to establish. We evaluated several growth factors, corticosteroids, rat serum, and a fibroblast feeder layer for their ability to produce and maintain a polarized epithelium of type II cells that secretes SP-A and SP-D into the apical medium. Type II cells were plated on a filter insert coated with an extracellular matrix and cultured at an air/liquid interface. KGF stimulated type II cell proliferation and secretion of SP-A and SP-D more than fibroblast growth factor 10 (FGF-10), hepatocyte growth factor (HGF), or heparin binding epidermal-like growth factor (HB-EGF). Cells cultured in the presence of KGF and rat serum with or without fibroblasts had high surfactant protein mRNA levels and exhibited a high level of SP-A and SP-D secretion. Dexamethasone inhibited type II cell proliferation but increased expression of SP-B. In the presence of KGF, rat serum, and dexamethasone, the mRNAs for the surfactant proteins were maintained at high levels. Secretion of SP-A and SP-D was found to be independent of phospholipid secretion.




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