Submitted on January 18, 2008
Revised on March 16, 2009
Accepted on May 4, 2009
15-lipoxygenase-1 induces expression and release of chemokines in cultured human lung epithelial cells
Cheng Liu1*, Dawei Xu2, Li Liu3, Frida Schain2, Asa Brunnstrom4, Magnus Bjorkholm2, Hans-Erik Claesson5, and Jan Sjöberg2
1 Karolinska University Hospital Solna
2 Karolinska University Hospital Solna and Karolinska Institutet
3 Shandong University
4 Orexo AB
5 Karolinska Institutet
* To whom correspondence should be addressed. E-mail: Cheng.Liu{at}ki.se.
15-lipoxygenase-1 (15-LOX-1) has been proposed to be involved in various physiological and pathophysiological activities such as inflammation, atherosclerosis, cell maturation, and tumorigenesis. Asthma and chronic obstructive pulmonary disease are associated with increased expression of 15-LOX-1 in bronchial epithelial cells, but the potential functions of 15-LOX-1 in airway epithelial cells have not been well clarified. To study the function of 15-LOX-1 in bronchial epithelial cells, we ectopically expressed 15-LOX-1 in the human lung epithelial cell line A549. We found that over-expression of 15-LOX-1 in A549 cells leads to increased release of the chemokines Mip-1
, RANTES and IP-10, and thereby to increased recruitment of immature dendritic cells, mast cells and activated T cells. These results suggest that an increased expression and activity of 15-LOX-1 in lung epithelial cells is a pro-inflammatory event in the pathogenesis of asthma and other inflammatory lung disorders.
