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Am J Physiol Lung Cell Mol Physiol (October 26, 2001). doi:10.1152/ajplung.00044.2001
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Articles in PresS, published online ahead of print October 24, 2001
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00044.2001
Submitted on February 2, 2001
Accepted on October 18, 2001

Cyclic mechanical stretch induces VEGF and FGF-2 expression in pulmonary vascular smooth muscle cells

Timothy P Quinn1*, Marielle Schlueter1, Scott J Soifer1, and Jorge A Gutierrez1

1 Pediatrics, University of California at San Francisco, San Francisco, CA, USA

* To whom correspondence should be addressed. E-mail: tpquinn{at}itsa.ucsf.edu.

Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2/bFGF) are involved in vascular development and angiogenesis. Pulmonary artery smooth muscle cells express VEGF and FGF-2, and are subjected to mechanical forces during pulsatile blood flow. The effect of stretch on growth factor expression in these cells is not well characterized. We investigated the effect of cyclic stretch on the expression of VEGF and FGF-2 in ovine pulmonary artery smooth muscle cells. Primary confluent cells from 6-week old lambs were cultured on flexible silicon membranes and subjected to cyclic biaxial stretch (1 Hz; 5%-25% stretch; 4 to 48 hours). Non-stretched cells served as controls. Expression of VEGF and FGF-2 was determined by Northern blot analysis. Cyclic stretch induced expression of both VEGF and FGF-2 mRNA in a time- and amplitude-dependent manner. Maximum expression was found at 24 hours and 15% stretch (VEGF: 1.8-fold; FGF-2: 1.9-fold). These results demonstrate that mechanical stretch regulates VEGF and FGF-2 gene expression, which could play a role in pulmonary vascular development or in post-natal pulmonary artery function or disease.




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