| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Internal Medicine, Korea University Ansan Hospital, Ansan, Korea, Republic of
2 Department of Internal Medicine, Korea University Guro Hospital, Seoul, Korea, Republic of
3 Department of Internal Medicine, Hallym University Kangnam Sacred Heart Hospital, Seoul, Korea, Republic of
4 Department of Clinical Pathology, Kangwon National University College of Medicine, Chuncheon, Korea, Republic of
5 Department of Internal Medicine, Korea University Anam Hospital, Seoul, Korea, Republic of
* To whom correspondence should be addressed. E-mail: yoosh47{at}unitel.co.kr.
Bacterial infections of the lung are known to induce inflammatory responses, which lead to mucus hypersecretion. Moreover, mucin synthesis in the airways has been reported to be regulated by neutrophilic inflammation-induced epidermal growth factor receptor (EGFR) expression and its activation. And, matrix metalloproteinases (MMPs), especially MMP-9, have been reported to promote the transmigration of activated neutrophils. In this study, we investigated the associations between lipopolysaccharide(LPS)-induced goblet cell(GC) metaplasia and the EGFR expression and the effects of MMP inhibitor (MMPI). Various concentrations of LPS were instilled into the tracheas of pathogen-free Sprague-Dawley rats, and airways were examined at different times after LPS instillation. To examine the role of MMP-9, rats were treated 3 days before LPS instillation and daily thereafter with MMPI. Neutrophilic infiltration, alcian blue/periodic acid-Schiff(AB/PAS) staining and immunohistochemical staining for MUC5AC, EGFR and MMP-9 were performed. The instillation of LPS increased AB/PAS and MUC5AC stainings in time- and dose-dependent manners, and treatment with MMPI significantly prevented GC metaplasia. The instillation of LPS into the trachea also induced neutrophilic infiltration, and EGFR and MMP-9 expression in the airway epithelium, and MMPI was found to significantly prevent neutrophil recruitment, GC metaplasia, EGFR and MMP-9 expression. This study demonstrates that the MMP-9 and EGFR cascades are associated with LPS-induced mucus hypersecretion.
This article has been cited by other articles:
|
|
 |
|
  H. S. Deshmukh, A. McLachlan, J. J. Atkinson, W. D. Hardie, T. R. Korfhagen, M. Dietsch, Y. Liu, P. Y. P. Di, S. C. Wesselkamper, M. T. Borchers, et al. Matrix Metalloproteinase-14 Mediates a Phenotypic Shift in the Airways to Increase Mucin Production Am. J. Respir. Crit. Care Med., November 1, 2009; 180(9): 834 - 845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Vanlaere and C. Libert Matrix Metalloproteinases as Drug Targets in Infections Caused by Gram-Negative Bacteria and in Septic Shock Clin. Microbiol. Rev., April 1, 2009; 22(2): 224 - 239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. S. Deshmukh, C. Shaver, L. M. Case, M. Dietsch, S. C. Wesselkamper, W. D. Hardie, T. R. Korfhagen, M. Corradi, J. A. Nadel, M. T. Borchers, et al. Acrolein-Activated Matrix Metalloproteinase 9 Contributes to Persistent Mucin Production Am. J. Respir. Cell Mol. Biol., April 1, 2008; 38(4): 446 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yoshida and R. M. Tuder Pathobiology of Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease Physiol Rev, July 1, 2007; 87(3): 1047 - 1082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Kim, M. H. Suk, D. W. Yoon, S. H. Lee, G. Y. Hur, K. H. Jung, H. C. Jeong, S. Y. Lee, S. Y. Lee, I. B. Suh, et al. Inhibition of matrix metalloproteinase-9 prevents neutrophilic inflammation in ventilator-induced lung injury Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L580 - L587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tesfaigzi Roles of Apoptosis in Airway Epithelia Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 537 - 547. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
