AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (August 10, 2007). doi:10.1152/ajplung.00054.2007
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
293/4/L941    most recent
00054.2007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Irwin, D.
Right arrow Articles by Majka, S. M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Irwin, D.
Right arrow Articles by Majka, S. M
Submitted on February 7, 2007
Accepted on August 1, 2007

Neonatal Lung Side Population Cells Demonstrate Endothelial Potential and are Altered in Response to Hyperoxia Induced Lung Simplification

David Irwin1, Karen Helm2, Nzali Campbell3, Masa Imamura4, Karen A. Fagan5, Julie Harral3, Michelle Carr3, Katharine A Young3, Dwight J. Klemm6, Sarah A. Gebb7, Edward C. Dempsey8, James West9, and Susan M Majka10*

1 Medicine, University of Colorado HSC, Denver, Colorado, United States
2 CanCer Center, UC-HSC, Denver, Colorado, United States
3 Medicine, UCDHSC, Denver, Colorado, United States
4 Medicine, UC-HSC, Denver, Colorado, United States
5 Pulmonary & Critical Care Medicine, University of Colorado Health Sciences Center, Denver, Colorado, United States
6 Cardiovascular Pulmonary Research/Pulmonary Critical Care, University of Colorado Health Sciences Center, Denver,, Colorado, United States
7 Cell Biology and Neuroscience, University of South Alabama, Mobile, Alabama, United States
8 CVP Lab/B-133, University of Colorado, Denver, Colorado, United States
9 UCHSC
10 Dept of Medicine, University of Colorado Health Sciences Center, Denver, Colorado, United States

* To whom correspondence should be addressed. E-mail: susan.majka{at}uchsc.edu.

Lung side population (SP) cells are resident lung precursor cells with both epithelial and mesenchymal potential that are believed to play a role in normal lung development and repair. Neonatal hyperoxic exposure impairs lung development leading to a long-term decrease in gas exchange surfaces. The hypothesis that lung SP cells are altered during impaired lung development has not been studied. To address this issue we characterized the endothelial potential of neonatal lung SP and subsets of lung SP from neonatal mice following hyperoxic exposure during room air recovery. Lung SP cells were isolated and sorted on the basis of their capacity to efflux Hoechst 33342. The lung SP was further sorted based on expression of Flk-1 and CD45. In vitro, both CD45pos/Flk-1pos and CD45neg/Flk-1pos bind isolectin B4 and incorporate LDL and form networks in matrigel, indicating that these populations have endothelial cell characteristics. Hyperoxic exposure of neonatal mice resulted in subtle changes in vascular and alveolar density on P13, which persisted with room air recovery to P41. During room air recovery a decrease in lung SP cells was detected in the hyperoxic exposed group on postnatal day 13 followed by an increase on day 41. Within this group the lung SP subpopulation of cells expressing CD45 increased on day 21 41 and 55. Here we show that lung SP cells demonstrate endothelial potential and that the population distribution changes in number as well as composition following hyperoxic exposure.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. M. Majka, M. Skokan, L. Wheeler, J. Harral, S. Gladson, E. Burnham, J. E. Loyd, K. R. Stenmark, M. Varella-Garcia, and J. West
Evidence for cell fusion is absent in vascular lesions associated with pulmonary arterial hypertension
Am J Physiol Lung Cell Mol Physiol, December 1, 2008; 295(6): L1028 - L1039.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.