| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Medicine, University of Colorado HSC, Denver, Colorado, United States
2 CanCer Center, UC-HSC, Denver, Colorado, United States
3 Medicine, UCDHSC, Denver, Colorado, United States
4 Medicine, UC-HSC, Denver, Colorado, United States
5 Pulmonary & Critical Care Medicine, University of Colorado Health Sciences Center, Denver, Colorado, United States
6 Cardiovascular Pulmonary Research/Pulmonary Critical Care, University of Colorado Health Sciences Center, Denver,, Colorado, United States
7 Cell Biology and Neuroscience, University of South Alabama, Mobile, Alabama, United States
8 CVP Lab/B-133, University of Colorado, Denver, Colorado, United States
9 UCHSC
10 Dept of Medicine, University of Colorado Health Sciences Center, Denver, Colorado, United States
* To whom correspondence should be addressed. E-mail: susan.majka{at}uchsc.edu.
Lung side population (SP) cells are resident lung precursor cells with both epithelial and mesenchymal potential that are believed to play a role in normal lung development and repair. Neonatal hyperoxic exposure impairs lung development leading to a long-term decrease in gas exchange surfaces. The hypothesis that lung SP cells are altered during impaired lung development has not been studied. To address this issue we characterized the endothelial potential of neonatal lung SP and subsets of lung SP from neonatal mice following hyperoxic exposure during room air recovery. Lung SP cells were isolated and sorted on the basis of their capacity to efflux Hoechst 33342. The lung SP was further sorted based on expression of Flk-1 and CD45. In vitro, both CD45pos/Flk-1pos and CD45neg/Flk-1pos bind isolectin B4 and incorporate LDL and form networks in matrigel, indicating that these populations have endothelial cell characteristics. Hyperoxic exposure of neonatal mice resulted in subtle changes in vascular and alveolar density on P13, which persisted with room air recovery to P41. During room air recovery a decrease in lung SP cells was detected in the hyperoxic exposed group on postnatal day 13 followed by an increase on day 41. Within this group the lung SP subpopulation of cells expressing CD45 increased on day 21 41 and 55. Here we show that lung SP cells demonstrate endothelial potential and that the population distribution changes in number as well as composition following hyperoxic exposure.
This article has been cited by other articles:
|
|
 |
|
  T. van Haaften, R. Byrne, S. Bonnet, G. Y. Rochefort, J. Akabutu, M. Bouchentouf, G. J. Rey-Parra, J. Galipeau, A. Haromy, F. Eaton, et al. Airway Delivery of Mesenchymal Stem Cells Prevents Arrested Alveolar Growth in Neonatal Lung Injury in Rats Am. J. Respir. Crit. Care Med., December 1, 2009; 180(11): 1131 - 1142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Johns and K. Yamaji-Kegan Unveiling cell phenotypes in lung vascular remodeling Am J Physiol Lung Cell Mol Physiol, December 1, 2009; 297(6): L1056 - L1058. [Full Text] [PDF]
|
|
|
|
|
|
S. Majka and D. F. Alvarez Neovascular capacity of endothelial progenitor cells in the adult pulmonary circulation Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L868 - L869. [Full Text] [PDF]
|
|
|
|
|
|
S. M. Majka, M. Skokan, L. Wheeler, J. Harral, S. Gladson, E. Burnham, J. E. Loyd, K. R. Stenmark, M. Varella-Garcia, and J. West Evidence for cell fusion is absent in vascular lesions associated with pulmonary arterial hypertension Am J Physiol Lung Cell Mol Physiol, December 1, 2008; 295(6): L1028 - L1039. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
