AJP - Lung AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (April 18, 2008). doi:10.1152/ajplung.00076.2007
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
294/6/L1233    most recent
00076.2007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adir, Y.
Right arrow Articles by Ridge, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adir, Y.
Right arrow Articles by Ridge, K. M.
Submitted on February 27, 2007
Accepted on April 9, 2008

Overexpression of the Na,K-ATPase {alpha}2 subunit improves lung liquid clearance during ventilation-induced lung injury

Yochai Adir1, Lynn C Welch2, Vidas Dumasius2, Phillip Factor3, Jacob Iasha Sznajder2, and Karen M. Ridge2*

1 Technion-Israel Institute of Technology, Israel
2 Pulmonary and Critical Care, Northwestern Unversity, Chicago, Illinois, United States
3 Medicine, Columbia University, New York, United States

* To whom correspondence should be addressed. E-mail: kridge{at}northwestern.edu.

Mechanical ventilation with high tidal volumes (HVT) impairs lung liquid clearance (LLC) and downregulates alveolar epithelial Na,K-ATPase. We have previously reported that the Na,K-ATPase {alpha}2 subunit contributes to LLC in normal rat lungs. Here we tested whether overexpression of Na,K-ATPase {alpha}2 subunit in the alveolar epithelium would increase clearance in a HVT model of lung injury. We infected rat lungs with a replication incompetent adenovirus that expresses Na,K-ATPase {alpha}2 subunit gene (Ad{alpha}2) 7 days prior to HVT mechanical ventilation. HVT ventilation decreased LLC by ~50% in untreated, sham and Adnull infected rats. Overexpression of Na,K-ATPase {alpha}2 subunit, prevented the decrease in clearance caused by HVT and was associated with significant increases in Na,K-ATPase {alpha}2 protein abundance and activity in peripheral lung Basolateral membrane fractions. Ouabain at 10-5 M, a concentration that inhibits the {alpha}2 but not the Na,K-ATPase {alpha}1, decreased LLC in Ad{alpha}2 infected rats to the same level as sham and Adnull infected lungs suggesting that the increased clearance in Ad{alpha}2 lungs was due to Na,K-ATPase {alpha}2 expression and activity. In summary, we provide evidence that augmentation of the Na,K-ATPase {alpha}2 subunit, via gene transfer, may accelerate LLC in the injured lung.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. N. Z. Yu, Z. P. Traylor, and I. C. Davis
Effect of ventilation pressure on alveolar fluid clearance and {beta}-agonist responses in mice
Am J Physiol Lung Cell Mol Physiol, October 1, 2009; 297(4): L785 - L793.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2008 by the American Physiological Society.