AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (July 21, 2006). doi:10.1152/ajplung.00086.2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
291/5/L1018    most recent
00086.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhao, M.
Right arrow Articles by Laubach, V. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhao, M.
Right arrow Articles by Laubach, V. E.
Submitted on March 9, 2006
Accepted on July 13, 2006

Alveolar Macrophage Activation is a Key Initiation Signal for Acute Lung Ischemia-Reperfusion Injury

Minqing Zhao1*, Lucas G Fernandez1, Allan Doctor2, Ashish K Sharma1, Alexander Zarbock3, Curtis G Tribble1, Irving L Kron1, and Victor E. Laubach1

1 Surgery, University of Virginia, Charlottesville, Virginia, United States
2 Pediatrics, University of Virginia, Charlottesville, Virginia, United States
3 Cardiovascular Research Center, University of Virginia, Charlottesville, Virginia, United States

* To whom correspondence should be addressed. E-mail: mz2v{at}virginia.edu.

Lung ischemia-reperfusion (IR) injury is a biphasic inflammatory process. Previous studies indicate that the later phase is neutrophil-dependent and that alveolar macrophages (AMs) likely contribute to the acute phase of lung IR injury. However, the mechanism is unclear. AMs become activated and produce various cytokines and chemokines in many inflammatory responses including transplantation. We hypothesize that AMs respond to IR by producing key cytokines and chemokines and that depletion of AMs would reduce cytokine/chemokine expression and lung injury after IR. To test this, using a buffer-perfused, isolated mouse lung model, we studied the impact of AM depletion by lipsome-clodronate on IR-induced lung dysfunction/injury and expression of cytokines/chemokines. IR caused a significant increase in pulmonary artery pressure, wet/dry weight ratio, vascular permeability and TNF-{alpha}, MCP-1 and MIP-2 expression as well as decreased pulmonary compliance, when compared to sham lungs. After AM depletion, the changes in each of these parameters between IR and sham groups were significantly attenuated. Thus AM depletion protects the lungs from IR-induced dysfunction and injury and significantly reduces cytokine/chemokine production. Protein expression of TNF-{alpha} and MCP-1 are positively correlated to IR-induced lung injury, and AMs are a major producer/initiator of TNF-{alpha}, MCP-1 and MIP-2. We conclude that AMs are an essential player in the initiation of acute lung IR injury.




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Chao, J. G. Wood, V. G. Blanco, and N. C. Gonzalez
The Systemic Inflammation of Alveolar Hypoxia Is Initiated by Alveolar Macrophage-Borne Mediator(s)
Am. J. Respir. Cell Mol. Biol., November 1, 2009; 41(5): 573 - 582.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. Germano, P. Blyszczuk, A. Valaperti, G. Kania, S. Dirnhofer, U. Landmesser, T. F. Luscher, L. Hunziker, H. Zulewski, and U. Eriksson
Prominin-1/CD133+ Lung Epithelial Progenitors Protect from Bleomycin-induced Pulmonary Fibrosis
Am. J. Respir. Crit. Care Med., May 15, 2009; 179(10): 939 - 949.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
C. L. Lau, Y. Zhao, J. Kim, I. L. Kron, A. Sharma, Z. Yang, V. E. Laubach, J. Linden, G. Ailawadi, and D. J. Pinsky
Enhanced fibrinolysis protects against lung ischemia-reperfusion injury.
J. Thorac. Cardiovasc. Surg., May 1, 2009; 137(5): 1241 - 1248.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
Z. Yang, A. K. Sharma, J. Linden, I. L. Kron, and V. E. Laubach
CD4+ T lymphocytes mediate acute pulmonary ischemia-reperfusion injury.
J. Thorac. Cardiovasc. Surg., March 1, 2009; 137(3): 695 - 702.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Z. Yang, A. K. Sharma, M. Marshall, I. L. Kron, and V. E. Laubach
NADPH Oxidase in Bone Marrow-Derived Cells Mediates Pulmonary Ischemia-Reperfusion Injury
Am. J. Respir. Cell Mol. Biol., March 1, 2009; 40(3): 375 - 381.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
D. M. Okamura, S. Pennathur, K. Pasichnyk, J. M. Lopez-Guisa, S. Collins, M. Febbraio, J. Heinecke, and A. A. Eddy
CD36 Regulates Oxidative Stress and Inflammation in Hypercholesterolemic CKD
J. Am. Soc. Nephrol., March 1, 2009; 20(3): 495 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. S. Farley, L. Wang, and S. Mehta
Septic pulmonary microvascular endothelial cell injury: role of alveolar macrophage NADPH oxidase
Am J Physiol Lung Cell Mol Physiol, March 1, 2009; 296(3): L480 - L488.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
J. Kohmoto, A. Nakao, R. Sugimoto, Y. Wang, J. Zhan, H. Ueda, and K. R. McCurry
Carbon monoxide-saturated preservation solution protects lung grafts from ischemia-reperfusion injury.
J. Thorac. Cardiovasc. Surg., October 1, 2008; 136(4): 1067 - 1075.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
N. Geudens, M. Van de Wouwer, B. M. Vanaudenaerde, R. Vos, C. Van De Wauwer, G. M. Verleden, E. Verbeken, T. Lerut, D. E. M. Van Raemdonck, and E. M. Conway
The lectin-like domain of thrombomodulin protects against ischaemia-reperfusion lung injury
Eur. Respir. J., October 1, 2008; 32(4): 862 - 870.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Eckle, L. Fullbier, A. Grenz, and H. K. Eltzschig
Usefulness of pressure-controlled ventilation at high inspiratory pressures to induce acute lung injury in mice
Am J Physiol Lung Cell Mol Physiol, October 1, 2008; 295(4): L718 - L724.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
W. Qiu, L. Zheng, H. Gu, D. Chen, and Y. Chen
Comparison between adult and infant lung injury in a rabbit ischemia-reperfusion model.
J. Thorac. Cardiovasc. Surg., August 1, 2008; 136(2): 352 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. A. Bem, A. W. Farnand, V. Wong, A. Koski, M. E. Rosenfeld, N. van Rooijen, C. W. Frevert, T. R. Martin, and G. Matute-Bello
Depletion of resident alveolar macrophages does not prevent Fas-mediated lung injury in mice
Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L314 - L325.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
L. M. Gazoni, V. E. Laubach, D. P. Mulloy, A. Bellizzi, E. B. Unger, J. Linden, P. I. Ellman, T. C. Lisle, and I. L. Kron
Additive protection against lung ischemia-reperfusion injury by adenosine A2A receptor activation before procurement and during reperfusion
J. Thorac. Cardiovasc. Surg., January 1, 2008; 135(1): 156 - 165.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. Forbes, M. Pickell, M. Foroughian, L.-J. Yao, J. Lewis, and R. Veldhuizen
Alveolar macrophage depletion is associated with increased surfactant pool sizes in adult rats
J Appl Physiol, August 1, 2007; 103(2): 637 - 645.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
L. M. Gazoni, C. G. Tribble, M. Q. Zhao, E. B. Unger, R. A. Farrar, P. I. Ellman, L. G. Fernandez, V. E. Laubach, and I. L. Kron
Pulmonary Macrophage Inhibition and Inhaled Nitric Oxide Attenuate Lung Ischemia-Reperfusion Injury
Ann. Thorac. Surg., July 1, 2007; 84(1): 247 - 253.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. K. Sharma, L. G. Fernandez, A. S. Awad, I. L. Kron, and V. E. Laubach
Proinflammatory response of alveolar epithelial cells is enhanced by alveolar macrophage-produced TNF-{alpha} during pulmonary ischemia-reperfusion injury
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L105 - L113.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. J. Fields, J. M. Bishai, W. Mitzner, and E. M. Wagner
Effects of pulmonary ischemia on lung morphology
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L254 - L258.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.