| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
2 Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
3 Department of Health Evaluation Sciences, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
4 Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Obstetrics and Gynecology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA
* To whom correspondence should be addressed. E-mail: jfloros{at}psu.edu.
Chronic airway inflammation caused by Pseudomonas aeruginosa is an important feature of cystic fibrosis (CF). Surfactant protein A (SP-A) enhances phagocytosis of P. aeruginosa. Two genes - SP-A1 and SP-A2, encode human SP-A. We hypothesized that genetically determined differences in the activity of SP-A1 and SP-A2 gene products exist. To test this, we studied association of a non-mucoid P. aeruginosa bacteria (ATCC 39018) with rat alveolar macrophages (AMs) in the presence or absence of insect cell expressed human SP-A variants. We used two trios, each consisting of SP-A1, SP-A2, and their co-expressed SP-A1/SP-A2 variants. We tested the 6A2 and 6A4 alleles (for SP-A1), the 1A0 and 1A alleles (for SP-A2), and their respective coexpressed SP-A1/SP-A2 gene products. After incubation of AMs with P. aeruginosa in the presence of the SP-A variants at 37°C for 1h, the cell-association of bacteria was assessed by light microscopy analysis. We found: a) depending on SP-A concentration and variant, SP-A2 variants significantly increased the cell-association more than the SP-A1 variants (the phagocytic index for SP-A1 was about 52-95% of the SP-A2 activity); b) co-expressed variants at certain concentrations were more active than single gene products; c) the phagocytic index for SP-A variants was about 18-41 % of the hSP-A from BAL. We conclude that human SP-A variants in vitro enhance association of P. aeruginosa with rat alveolar macrophages differentially and in a concentration dependent manner, with SP-A2 variants having a higher activity compared to SP-A1 variants.
This article has been cited by other articles:
|
|
 |
|
  S. R. Bruce, C. L. Atkins, G. N. Colasurdo, and J. L. Alcorn Respiratory syncytial virus infection alters surfactant protein A expression in human pulmonary epithelial cells by reducing translation efficiency Am J Physiol Lung Cell Mol Physiol, October 1, 2009; 297(4): L559 - L567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wang, X. Guo, P. Silveyra, S. R. Kimball, and J. Floros Cap-independent translation of human SP-A 5'-UTR variants: a double-loop structure and cis-element contribution Am J Physiol Lung Cell Mol Physiol, April 1, 2009; 296(4): L635 - L647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Mikerov, T. M. Umstead, X. Gan, W. Huang, X. Guo, G. Wang, D. S. Phelps, and J. Floros Impact of ozone exposure on the phagocytic activity of human surfactant protein A (SP-A) and SP-A variants Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L121 - L130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. R. S. Tagaram, G. Wang, T. M. Umstead, A. N. Mikerov, N. J. Thomas, G. R. Graff, J. C. Hess, M. J. Thomassen, M. S. Kavuru, D. S. Phelps, et al. Characterization of a human surfactant protein A1 (SP-A1) gene-specific antibody; SP-A1 content variation among individuals of varying age and pulmonary health Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1052 - L1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Mikerov, G. Wang, T. M. Umstead, M. Zacharatos, N. J. Thomas, D. S. Phelps, and J. Floros Surfactant Protein A2 (SP-A2) Variants Expressed in CHO Cells Stimulate Phagocytosis of Pseudomonas aeruginosa More than Do SP-A1 Variants Infect. Immun., March 1, 2007; 75(3): 1403 - 1412. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
