AJP - Lung Columbus Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (December 14, 2001). doi:10.1152/ajplung.00143.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/L1151    most recent
00143.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liedtke, C. M
Right arrow Articles by Cole, T. S
Right arrow Search for Related Content
PubMed
Right arrow Articles by Liedtke, C. M
Right arrow Articles by Cole, T. S

Articles in PresS, published online ahead of print December 14, 2001
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00143.2001
Submitted on April 25, 2001
Accepted on December 11, 2001

Modulation of Na-K-2Cl cotransport by intracellular Cl and protein kinase C-{delta} in Calu-3 cells

Carole M Liedtke1*, Robert Papay1, and Thomas S Cole1

1 Pediatrics, Case Western Reserve University, Cleveland, OH, USA

* To whom correspondence should be addressed. E-mail: CXL7{at}PO.CWRU.EDU.

In this study, we test the hypothesis that intracellular Cl (Cli) regulates activity of PKC-{delta} and, thus, activation of Na-K-Cl cotransport (NKCC1) in a Calu-3 cell line. An {alpha}1-adrenergic agonist methoxamine (MOX) and hypertonic sucrose increased Cli and increased or decreased intracellular volume (Vi), respectively, without changing [Cl]i. Titrating [Cl]i from 20-140 mM in nystatin-permeabilized cell monolayers did not affect baseline activity of PKC -{delta} or -{zeta} or rottlerin-sensitive NKCC1. At 200 mM Cl, rottlerin-sensitive NKCC1 was activated and PKC isotypes were localized predominantly to a particulate fraction. MOX induced a biphasic increase in NKCC1 activity and in activity of PKC-{delta} and particulate localization of PKC-{delta} and-{zeta}. Activity of NKCC1 and PKC-{delta} decreased with increasing Cli from 20 to 80 mM Cli then increased at 140-200 mM Cli apparently as an additive effect to high [Cl]i. Rottlerin inhibited the effects of MOX, indicating that PKC-{delta} was required for activation of NKCC1. The results indicate that, in airway epithelial cells, a Cli electrochemical gradient alone is not sufficient to stimulate NKCC1 activity; rather elevated activity of PKC-{delta} is necessary. Further, high Cli induces a subcellular redistribution of PKC-{delta} resulting in increased enzyme activity.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Q. van der Merwe, F. Moreau, and W. K. MacNaughton
Protease-activated receptor-2 stimulates intestinal epithelial chloride transport through activation of PLC and selective PKC isoforms
Am J Physiol Gastrointest Liver Physiol, June 1, 2009; 296(6): G1258 - G1266.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
T. Matsuno, Y. Ito, T. Ohashi, M. Morise, N. Takeda, K. Shimokata, K. Imaizumi, H. Kume, and Y. Hasegawa
Dual Pathway Activated by tert-Butyl Hydroperoxide in Human Airway Anion Secretion
J. Pharmacol. Exp. Ther., November 1, 2008; 327(2): 453 - 464.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
T. Ohashi, Y. Ito, T. Matsuno, S. Sato, K. Shimokata, and H. Kume
Paradoxical Effects of Hydrogen Peroxide on Human Airway Anion Secretion
J. Pharmacol. Exp. Ther., July 1, 2006; 318(1): 296 - 303.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
I. C. Del Castillo, M. Fedor-Chaiken, J. C. Song, V. Starlinger, J. Yoo, K. S. Matlin, and J. B. Matthews
Dynamic regulation of Na+-K+-2Cl- cotransporter surface expression by PKC-{epsilon} in Cl--secretory epithelia
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1332 - C1343.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. Walcott, A. Birzgalis, L. C. Moore, and P. R. Brink
Fluid secretion and the Na+-K+-2Cl- cotransporter in mouse exorbital lacrimal gland
Am J Physiol Cell Physiol, October 1, 2005; 289(4): C860 - C867.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. D. Smallwood, B. S. Hausman, X. Wang, and C. M. Liedtke
Involvement of NH2 terminus of PKC-{delta} in binding to F-actin during activation of Calu-3 airway epithelial NKCC1
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C906 - C912.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. H. Abdullah, J. T. Bundy, C. Ehre, and C. W. Davis
Mucin secretion and PKC isoforms in SPOC1 goblet cells: differential activation by purinergic agonist and PMA
Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L149 - L160.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. M. Liedtke, M. Hubbard, and X. Wang
Stability of actin cytoskeleton and PKC-delta binding to actin regulate NKCC1 function in airway epithelial cells
Am J Physiol Cell Physiol, February 1, 2003; 284(2): C487 - C496.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2001 by the American Physiological Society.