AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (August 1, 2003). doi:10.1152/ajplung.00145.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/6/L1270    most recent
00145.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ameredes, B. T.
Right arrow Articles by Choi, A. M.K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ameredes, B. T.
Right arrow Articles by Choi, A. M.K.
Submitted on May 9, 2003
Accepted on July 31, 2003

Low-dose Carbon Monoxide Reduces Airway Hyperresponsiveness in Mice

Bill T. Ameredes1*, Leo E. Otterbein2, Lauryn K. Kohut1, Amber L. Gligonic1, William J. Calhoun1, and Augustine M.K. Choi2

1 Asthma, Allergy, and Airway Research Center, University of Pittsburgh, Pittsburgh, PA, USA; Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA, USA
2 Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, Pittsburgh, PA, USA

* To whom correspondence should be addressed. E-mail: ameredesbt{at}msx.upmc.edu.

Carbon monoxide (CO) in expired gas has been shown to be elevated with asthma, however its function is not known, and there is some potential that it may serve a bronchoprotective role to decrease airway hyperresponsiveness (AHR). Thus, the ability of carbon monoxide to reverse methacholine (MCh) induced bronchoconstriction was evaluated in C57Bl6 (C57) and A/J mice, with and without airway inflammation produced by ovalbumin (OVA). Acutely administered CO (1% in air, 10 min.) reduced MCh-driven increases in lung resistance in OVA-challenged C57 mice, by an average of 50% (14.5 to 7.1 cm H2O x ml-1 x sec-1), while no effect was observed in naive C57 mice, or OVA-challenged C57 mice inhaling air, alone. Acutely inhaled CO (500 ppm = 0.05%, for 10 min.) reduced MCh-induced airway reactivity (AR) by 20-60% in airway hyperresponsive naive A/J mice, while repeated 10-min. administrations of 500 ppm CO over a five day period decreased AR by 50%. Repeated administration of low-dose CO (250 [0.025%] and 500 ppm, 1 hour/day, 5 days)] to A/J mice with airway inflammation likewise resulted in a drop of AR by 50%, as compared to those not receiving CO. Inhibition of guanylyl cyclase/cGMP using 1H-[1,2,4] Oxydiazolo[4,3-a]quinoxalin-1-one (ODQ), or a competitive inhibitor, Rp-8Br-cGMPs, resulted in inhibition of the effect of CO on AHR, suggesting that the effects of CO were mediated through this mechanism. These results indicate that low-dose CO can effectively reverse AHR in the presence and absence of airway inflammation in mice, and suggest a potential role for CO in the modulation of AHR.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Nemzek, C. Fry, and O. Abatan
Low-dose carbon monoxide treatment attenuates early pulmonary neutrophil recruitment after acid aspiration
Am J Physiol Lung Cell Mol Physiol, April 1, 2008; 294(4): L644 - L653.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
L. E. Fredenburgh, M. A. Perrella, and S. A. Mitsialis
The Role of Heme Oxygenase-1 in Pulmonary Disease
Am. J. Respir. Cell Mol. Biol., February 1, 2007; 36(2): 158 - 165.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. W. Ryter, D. Morse, and A. M. K. Choi
Carbon Monoxide and Bilirubin: Potential Therapies for Pulmonary/Vascular Injury and Disease
Am. J. Respir. Cell Mol. Biol., February 1, 2007; 36(2): 175 - 182.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. J. Canning
Reflex regulation of airway smooth muscle tone
J Appl Physiol, September 1, 2006; 101(3): 971 - 985.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Y. Suh, Y. Jin, A.-K. Yi, X. M. Wang, and A. M. K. Choi
CCAAT/Enhancer-Binding Protein Mediates Carbon Monoxide-Induced Suppression of Cyclooxygenase-2
Am. J. Respir. Cell Mol. Biol., August 1, 2006; 35(2): 220 - 226.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. W. Ryter, J. Alam, and A. M. K. Choi
Heme Oxygenase-1/Carbon Monoxide: From Basic Science to Therapeutic Applications
Physiol Rev, April 1, 2006; 86(2): 583 - 650.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Papapetropoulos, D. C. M. Simoes, G. Xanthou, C. Roussos, and C. Gratziou
Soluble guanylyl cyclase expression is reduced in allergic asthma
Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L179 - L184.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. Morse and A. M. K. Choi
Heme Oxygenase-1: From Bench to Bedside
Am. J. Respir. Crit. Care Med., September 15, 2005; 172(6): 660 - 670.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. B. Moerloose, R. A. Pauwels, and G. F. Joos
Short-Term Cigarette Smoke Exposure Enhances Allergic Airway Inflammation in Mice
Am. J. Respir. Crit. Care Med., July 15, 2005; 172(2): 168 - 172.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. T. Ameredes, J. M. Sethi, H.-L. Liu, A. M. K. Choi, and W. J. Calhoun
Enhanced nitric oxide production associated with airway hyporesponsiveness in the absence of IL-10
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L868 - L873.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. P. Carter, C. Garat, and M. Imamura
Continual emerging roles of HO-1: protection against airway inflammation
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L24 - L25.
[Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. N. Melgert, D. S. Postma, M. Geerlings, M. A. Luinge, P. A. Klok, B. W. A. van der Strate, H. A. M. Kerstjens, W. Timens, and M. N. Hylkema
Short-Term Smoke Exposure Attenuates Ovalbumin-Induced Airway Inflammation in Allergic Mice
Am. J. Respir. Cell Mol. Biol., June 1, 2004; 30(6): 880 - 885.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.