| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print December 20, 2001
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00148.2001
Submitted on April 26, 2001
Accepted on December 18, 2001
1 Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USA; Research Service, Omaha VA Medical Center, Omaha, NE, USA
2 Internal Medicine, University of Nebraska Medical Center, Omaha, NE, USA
3 Research Service, Omaha VA Medical Center, Omaha, NE, USA
* To whom correspondence should be addressed. E-mail: jspurzem{at}unmc.edu.
Bronchial epithelial cell migration is required for the repair of damaged airway epithelium. We hypothesized that bronchial epithelial cell migration during wound repair is influenced by cAMP and the activity of its cyclic nucleotide dependent protein kinase, PKA. We found that when confluent monolayers of bronchial epithelial cells are wounded, an increase in PKA activity occurs. Augmentation of PKA activity with a cell permeable analog of cAMP, dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), isoproterenol, or a phosphodiesterase inhibitor accelerated migration of normal bronchial epithelial cells in in vitro wound closure assays and Boyden chamber migration assays. A role for PKA activity was also confirmed with a PKA inhibitor, KT5720, which reduced stimulated migration. Augmentation of PKA activity reduced the levels of active Rho and the formation of focal adhesions. These studies suggest PKA activation modulates Rho activity, migration mechanisms and thus bronchial epithelial repair mechanisms.
This article has been cited by other articles:
|
|
 |
|
  G D Perkins, F Gao, and D R Thickett In vivo and in vitro effects of salbutamol on alveolar epithelial repair in acute lung injury Thorax, March 1, 2008; 63(3): 215 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Slager, D. S. Allen-Gipson, A. Sammut, A. Heires, J. DeVasure, S. Von Essen, D. J. Romberger, and T. A. Wyatt Hog barn dust slows airway epithelial cell migration in vitro through a PKC{alpha}-dependent mechanism Am J Physiol Lung Cell Mol Physiol, December 1, 2007; 293(6): L1469 - L1474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Puchelle, J.-M. Zahm, J.-M. Tournier, and C. Coraux Airway Epithelial Repair, Regeneration, and Remodeling after Injury in Chronic Obstructive Pulmonary Disease Proceedings of the ATS, November 1, 2006; 3(8): 726 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Milan, C. Charalambous, R. Elhag, T. C. Chen, W. Li, S. Guan, F. M. Hofman, and R. Zidovetzki Multiple signaling pathways are involved in endothelin-1-induced brain endothelial cell migration Am J Physiol Cell Physiol, July 1, 2006; 291(1): C155 - C164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Allen-Gipson, J. Wong, J. R. Spurzem, J. H. Sisson, and T. A. Wyatt Adenosine A2A receptors promote adenosine-stimulated wound healing in bronchial epithelial cells Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L849 - L855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Schnackenberg, S. M. Jones, C. Pate, B. Shank, L. Sessions, L. M. Pittman, L. E. Cornett, and R. C. Kurten The beta-agonist isoproterenol attenuates EGF-stimulated wound closure in human airway epithelial cells Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L485 - L491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Pullar and R. R. Isseroff The {beta}2-adrenergic receptor activates pro-migratory and pro-proliferative pathways in dermal fibroblasts via divergent mechanisms J. Cell Sci., February 1, 2006; 119(3): 592 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. N. Chorley, Y. Li, S. Fang, J.-A. Park, and K. B. Adler (R)-Albuterol Elicits Antiinflammatory Effects in Human Airway Epithelial Cells via iNOS Am. J. Respir. Cell Mol. Biol., January 1, 2006; 34(1): 119 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Desai, A. M. Aryal, B. Ceacareanu, A. Hassid, and C. M. Waters RhoA and Rac1 are both required for efficient wound closure of airway epithelial cells Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1134 - L1144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Hopkins, M. Bruewer, G. T. Brown, A. A. Pineda, J. J. Ha, L. M. Winfree, S. V. Walsh, B. A. Babbin, and A. Nusrat Epithelial cell spreading induced by hepatocyte growth factor influences paxillin protein synthesis and posttranslational modification Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G886 - G898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Goncharova, C. K. Billington, C. Irani, A. V. Vorotnikov, V. A. Tkachuk, R. B. Penn, V. P. Krymskaya, and R. A. Panettieri Jr. Cyclic AMP-Mobilizing Agents and Glucocorticoids Modulate Human Smooth Muscle Cell Migration Am. J. Respir. Cell Mol. Biol., July 1, 2003; 29(1): 19 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.A. Wyatt, J.H. Sisson, M.A. Forget, R.G. Bennett, F.G. Hamel, and J.R. Spurzem Relaxin Stimulates Bronchial Epithelial Cell PKA Activation, Migration, and Ciliary Beating Experimental Biology and Medicine, December 1, 2002; 227(11): 1047 - 1053. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
