| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Environmental Medicine, University of Rochester, Rochester, NY, USA; Departments of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA, USA
2 Departments of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA, USA; Department of Environmental Medicine, University of Rochester, Rochester, NY, USA
3 Departments of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA, USA
4 Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY, USA
5 Departments of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA, USA; Departments of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA, USA; Department of Environmental Medicine, University of Rochester, Rochester, NY, USA
* To whom correspondence should be addressed. E-mail: brs2{at}pitt.edu.
The composition of the conducting airway epithelium varies significantly along the proximal to distal axis, with that of the tracheal epithelium exhibiting the greatest complexity. A number of progenitor cells have been proposed to contribute to the maintenance of this cellular diversity both in the steady-state and in response to injury. However, individual roles for each progenitor cell type are poorly defined in vivo. The present study was undertaken to investigate the hypothesis that basal cells represent a multipotent progenitor cell type for renewal of the injured tracheal epithelium. GSIB4-reactive basal cells of the steady-state mouse tracheal epithelium were heterogeneous for expression of cytokeratin 14 (K14). To understand their contribution to epithelial repair mice were exposed to naphthalene to induce airway injury and depletion of the secretory cell progenitor pool. Injury resulted in a rapid induction of K14 expression among the majority of GSIB4-reactive cells and associated hyperplasia of basal cells. Restoration of depleted secretory cells occurred after 6 days of recovery and was associated with regression of the basal cell hyperplasia suggesting a progenitor-progeny relationship. Multipotent differentiation of basal cells was confirmed using a bitransgenic ligand-regulated Cre-loxP reporter approach in which expression of a ubiquitously expressed LacZ reporter was activated within K14-expressing progenitor cells during airway repair. Using this approach it was determined that K14-expressing cells include subsets capable of either multipotent or unipotent differentiation in vivo. We conclude that basal cells have the capacity for restoration of a fully differentiated epithelium.
This article has been cited by other articles:
|
|
 |
|
  E.L. Rawlins, T. Okubo, J. Que, Y. Xue, C. Clark, X. Luo, and B.L.M. Hogan Epithelial Stem/Progenitor Cells in Lung Postnatal Growth, Maintenance, and Repair Cold Spring Harb Symp Quant Biol, November 26, 2008; (2008) sqb.2008.73.037v2. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Coraux, J. Roux, T. Jolly, and P. Birembaut Epithelial Cell-Extracellular Matrix Interactions and Stem Cells in Airway Epithelial Regeneration Proceedings of the ATS, August 15, 2008; 5(6): 689 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Wagh, E. Roan, K. E. Chapman, L. P. Desai, D. A. Rendon, E. C. Eckstein, and C. M. Waters Localized elasticity measured in epithelial cells migrating at a wound edge using atomic force microscopy Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L54 - L60. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Kim Paving the road for lung stem cell biology: bronchioalveolar stem cells and other putative distal lung stem cells Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1092 - L1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Heguy, B.-G. Harvey, P. L. Leopold, I. Dolgalev, T. Raman, and R. G. Crystal Responses of the human airway epithelium transcriptome to in vivo injury Physiol Genomics, April 24, 2007; 29(2): 139 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Reynolds, H. Shen, P. R. Reynolds, T. Betsuyaku, J. M. Pilewski, F. Gambelli, M. DeGuiseppe, L. A. Ortiz, and B. R. Stripp Molecular and functional properties of lung SP cells Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L972 - L983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Giangreco, K. R. Groot, and S. M. Janes Lung Cancer and Lung Stem Cells: Strange Bedfellows? Am. J. Respir. Crit. Care Med., March 15, 2007; 175(6): 547 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Rawlins and B. L. M. Hogan Epithelial stem cells of the lung: privileged few or opportunities for many? Development, July 1, 2006; 133(13): 2455 - 2465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Stripp and S. D. Reynolds Bioengineered Lung Epithelium: Implications for Basic and Applied Studies in Lung Tissue Regeneration Am. J. Respir. Cell Mol. Biol., February 1, 2005; 32(2): 85 - 86. [Full Text] [PDF]
|
|
|
|
|
|
C. M. Evans, O. W. Williams, M. J. Tuvim, R. Nigam, G. P. Mixides, M. R. Blackburn, F. J. DeMayo, A. R. Burns, C. Smith, S. D. Reynolds, et al. Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen-Challenged Mice Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 382 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Pitt and L. A. Ortiz Stem cells in lung biology Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L621 - L623. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
