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1 Department of Otorhinolaryngology-Head and Neck Surgery, Konkuk University College of Medicine, Seoul, United States
2 Department of Otorhinolaryngology, 3the Airway Mucus Institute,, Seoul, Korea, Republic of
3 Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea, Republic of
4 Department of Otorhinolaryngology,the Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Korea, Republic of
5 Department of Otorhinolaryngology-Head and Neck Surgery, Konkuk University College of Medicine, Seoul, United States; Seoul, Korea, Republic of
6 seoul, Korea, Republic of; Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea, Republic of
7 Department of Otorhinolaryngology, the Airway Mucus Institute, the Brain Korea 21 Project for Medical Science, Biomolecule Sec, Yonsei University College of Medicine, Seoul, Korea, Republic of
* To whom correspondence should be addressed. E-mail: jhyoon{at}yumc.yonsei.ac.kr.
Surfactant proteins (SPs) designated SP-A, SP-B, SP-C and SP-D, play an important role in surfactant metabolism and host defense mechanism in the lung. This study investigates expression of the different SP types in human nasal mucosa and cultured normal human nasal epithelial (NHNE) cells, and whether the expression of SP mRNA is influenced by the degree of mucociliary differentiation. Reverse transcription-polymerase chain reaction (RT-PCR) was performed with mRNA from cultured NHNE cells and nasal mucosa. Immunohistochemical staining for SPs was performed on nasal mucosa specimens. Western blot analysis was performed on cell lysates from cultured NHNE cells. SP-A2, SP-B and SP-D mRNAs were expressed in normal NHNE cells and human nasal mucosa. SPs were localized in ciliated cells of the surface epithelium and serous acini of the submucosal glands. SP-A, SP-B and SP-D proteins were expressed in cultured NHNE cells. The degree of mucociliary differentiation influenced expression of the SP gene. We demonstrate that SP-A, SP-B and SP-D are expressed in human nasal mucosa and cultured NHNE cells. Further study of the functional role of SPs in the upper airway is required.
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