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1 Department of Surgery and Development Biology Program, Childrens Hospital Los Angeles Research Institute, Los Angeles, CA, USA
2 Research Immunology, Childrens Hospital Los Angeles, Los Angeles, CA, USA
* To whom correspondence should be addressed. E-mail: bdriscoll{at}chla.usc.edu.
Alveolar epithelial type 2 cells (AEC2) isolated from hyperoxia-treated animals exhibit increases in both proliferation and DNA damage in response to culture. AEC2 express the zonula adherens proteins E-cadherin, 
-, 
- and 
-catenin, desmoglein and pp120 as demonstrated by western blotting. Immunohistochemical analysis of cultured AEC2 showed expression of E-cadherin on cytoplasmic membranes varying from strongly to weakly staining. When cultured AEC2 placed in suspension were labeled with fluorescent-tagged antibodies to E-cadherin, cells could be sorted into at least two sub-populations, either dim or brightly staining for this marker. Using antibody to E-cadherin bound to magnetic beads, cells were physically separated into E-cadherin positive and negative subpopulations, which were then analyzed for differences in proliferation and DNA damage. The E-cadherin positive subpopulation contained the majority of damaged cells, was quiescent and expressed low levels of telomerase activity, while the E-cadherin negative subpopulation was undamaged, proliferative and expressed high levels of telomerase activity.
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