| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Physiology, University of South Alabama, Mobile, AL, USA
2 Ninewells Hospital Department of Maternal and Child Health Sciences, Dundee University, Dundee, Scotland, United Kingdom
* To whom correspondence should be addressed. E-mail: sballard{at}usouthal.edu.
cAMP-elevating agents such as forskolin and vasoactive intestinal peptide (VIP) induce liquid secretion by tracheobronchial submucosal glands. This pathway is thought to be CFTR-dependent and thus defective in cystic fibrosis; however, the ionic mechanism that drives this secretion process is incompletely understood. To better define this mechanism, we studied the effects of ion transport inhibitors on the forskolin-induced liquid secretion response (Jv) of porcine distal bronchi. The forskolin-induced Jv was driven by a combination of bumetanide-sensitive Cl- secretion and DIDS-sensitive HCO3- secretion. When C1- secretion was inhibited with bumetanide, Na+/H+ exchange (NHE)-dependent HCO3- secretion was apparently induced to compensate for the loss of C1- secretion. The forskolin-induced Jv was significantly inhibited by the anion channel blockers NPPB, DPC, and glibenclamide. We conclude that the forskolin-induced Jv shares many characteristics of cholinergically-induced secretion except for the presence of a DIDS-sensitive component. While the identity of the DIDS-sensitive component is unclear, we speculate that it represents a basolateral membrane Na+-HCO3- cotransporter or a Na+-dependent anion exchanger, which could account for transepithelial HCO3- secretion.
This article has been cited by other articles:
|
|
 |
|
  C. S. Rogers, W. M. Abraham, K. A. Brogden, J. F. Engelhardt, J. T. Fisher, P. B. McCray Jr., G. McLennan, D. K. Meyerholz, E. Namati, L. S. Ostedgaard, et al. The porcine lung as a potential model for cystic fibrosis Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L240 - L263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Verkman From the farm to the lab: the pig as a new model of cystic fibrosis lung disease Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L238 - L239. [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Ianowski, J. Y. Choi, J. J. Wine, and J. W. Hanrahan Mucus secretion by single tracheal submucosal glands from normal and cystic fibrosis transmembrane conductance regulator knockout mice J. Physiol., April 1, 2007; 580(1): 301 - 314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Liu, M. Luo, L. Zhang, W. Ding, Z. Yan, and J. F. Engelhardt Bioelectric Properties of Chloride Channels in Human, Pig, Ferret, and Mouse Airway Epithelia Am. J. Respir. Cell Mol. Biol., March 1, 2007; 36(3): 313 - 323. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
