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1 Klinik fuer Kinder und Jugendliche, Universitaet Erlangen-Nuernberg, Erlangen, Germany
2 Institut fuer Pathologie, Universitaet Giessen, Giessen, Germany
* To whom correspondence should be addressed. E-mail: Wolfgang.Rascher{at}kinder.med.uni-erlangen.de.
The aim was to identify cell types involved in the anti-inflammatory effect of ventilation with perfluorocarbon in vivo. Fifteen anesthetized, surfactant depleted piglets received either aerosolized perfluorocarbon (Aerosol-PFC), partial liquid ventilation (FRC-PLV), or intermittent mandatory ventilation (control). After laser-assisted microdissection of different lung cell types, mRNA expression of IL-8 and ICAM-1 was determined using TaqMan real time PCR normalized to hypoxanthine-guanine-phosphoribosyl-transferase (HPRT). IL-8 mRNA expression (mean ± SEM; control vs. Aerosol-PFC) was: 356 ± 142 copies IL-8 mRNA / copy HPRT mRNA vs. 3.5 ± 1.8 in alveolar macrophages (p<0.01); 208 ± 108 vs. 2.7 ± 0.8 in bronchiolar epithelial cells (p<0.05); 26 ± 11 vs. 0.7 ± 0.2 in alveolar septum cells (p<0.01); 2.8 ± 1.0 vs. 0.8 ± 0.4 in bronchiolar smooth muscle cells (p<0.05) and 1.1 ± 0.4 vs. 0.2 ± 0.05 in vascular smooth muscle cells (p<0.05). With FRC-PLV, IL-8/HPRT mRNA expression was significantly lower in macrophages, bronchiolar epithelial and vascular smooth muscle cells. ICAM-1 mRNA expression in vascular endothelial cells remained unchanged. Predominantly alveolar macrophages and bronchiolar epithelial cells were involved in the inflammatory pulmonary process. The anti-inflammatory effect of Aerosol-PFC was most pronounced.
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