| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print November 16, 2001
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00206.2001
Submitted on June 6, 2001
Accepted on November 10, 2001
B Activation and Proliferation in Human Airway Smooth Muscle
1 Internal Medicine Research, Carolinas Medical Center, Charlotte, NC, USA
2 Division of Respiratory, Critical Care and Occupational (Pulmonary) Medicine, University of Utah, Salt Lake City, UT, USA
3 Montana State University, Veterinary Molecular Biology, Bozeman, MT, USA
4 Division of Pulmonary Diseases, Dept. of Pediatrics, Duke University Medical Center, Durham, NC, USA
* To whom correspondence should be addressed. E-mail: tkennedy{at}carolinas.org.
Evidence is rapidly accumulating that low-activity NAD(P)H oxidases homologous to that in phagocytic cells generate reactive oxygen species (ROS) as signaling intermediates in both endothelium and vascular smooth muscle. We therefore explored the possibility of such an oxidase regulating growth of airway smooth muscle. Proliferation of human airway smooth muscle (AWSM) cells in culture was inhibited by the antioxidants catalase and N-acetylcysteine, and by the flavoprotein inhibitor diphenylene iodonium (DPI). Membranes prepared from human AWSM cells generated superoxide, measured by SOD-inhibitable lucigenin chemiluminescence, with a distinct preference for NADH instead of NADPH as substrate. Chemiluminescence was also inhibited by DPI, suggesting the presence of a flavoprotein containing oxidase generating superoxide as a signaling molecule for cell growth. Examination of human AWSM cells by RT-PCR consistently demonstrated transcripts with sequences identical to those reported for p22phox. Transfection with p22phox antisense oligonucleotides reduced human AWSM proliferation. Inhibition of NAD(P)H oxidase activity with DPI prevented serum-induced activation of nuclear factor- B (NF-B), and over-expression of a superrepressor for of the NF-B inhibitor IB-
significantly reduced human AWSM growth. These findings suggest that an NAD(P)H oxidase containing p22phox regulates growth-factor responsive human AWSM proliferation, and that the oxidase signals in part through activation of the prototypical redox-regulated transcription factor NF-B.
This article has been cited by other articles:
|
|
 |
|
  J. K. Bentley and M. B. Hershenson Airway Smooth Muscle Growth in Asthma: Proliferation, Hypertrophy, and Migration Proceedings of the ATS, January 1, 2008; 5(1): 89 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chitano, L. Wang, S. N. Mason, R. L. Auten, E. N. Potts, W. M. Foster, A. Sturrock, T. P. Kennedy, J. R. Hoidal, and T. M. Murphy Airway smooth muscle relaxation is impaired in mice lacking the p47phox subunit of NAD(P)H oxidase Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L139 - L148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chachami, A. Hatziefthimiou, P. Liakos, M. G. Ioannou, G. K. Koukoulis, S. Bonanou, P.-A. Molyvdas, G. Simos, and E. Paraskeva Exposure of differentiated airway smooth muscle cells to serum stimulates both induction of hypoxia-inducible factor-1{alpha} and airway responsiveness to ACh Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L913 - L922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Gauss, L. K. Nelson-Overton, D. W. Siemsen, Y. Gao, F. R. DeLeo, and M. T. Quinn Role of NF-{kappa}B in transcriptional regulation of the phagocyte NADPH oxidase by tumor necrosis factor-{alpha} J. Leukoc. Biol., September 1, 2007; 82(3): 729 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Donato, I. Eskurza, A. E. Silver, A. S. Levy, G. L. Pierce, P. E. Gates, and D. R. Seals Direct Evidence of Endothelial Oxidative Stress With Aging in Humans: Relation to Impaired Endothelium-Dependent Dilation and Upregulation of Nuclear Factor-{kappa}B Circ. Res., June 8, 2007; 100(11): 1659 - 1666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sturrock, T. P. Huecksteadt, K. Norman, K. Sanders, T. M. Murphy, P. Chitano, K. Wilson, J. R. Hoidal, and T. P. Kennedy Nox4 mediates TGF-beta1-induced retinoblastoma protein phosphorylation, proliferation, and hypertrophy in human airway smooth muscle cells Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1543 - L1555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bedard and K.-H. Krause The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology Physiol Rev, January 1, 2007; 87(1): 245 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. ten Freyhaus, M. Huntgeburth, K. Wingler, J. Schnitker, A. T. Baumer, M. Vantler, M. M. Bekhite, M. Wartenberg, H. Sauer, and S. Rosenkranz Novel Nox inhibitor VAS2870 attenuates PDGF-dependent smooth muscle cell chemotaxis, but not proliferation Cardiovasc Res, July 15, 2006; 71(2): 331 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sturrock, B. Cahill, K. Norman, T. P. Huecksteadt, K. Hill, K. Sanders, S. V. Karwande, J. C. Stringham, D. A. Bull, M. Gleich, et al. Transforming growth factor-beta1 induces Nox4 NAD(P)H oxidase and reactive oxygen species-dependent proliferation in human pulmonary artery smooth muscle cells Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L661 - L673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Park, R. E. Ferrell, J.-J. Park, J. M. Hagberg, D. A. Phares, J. M. Jones, and M. D. Brown NADPH oxidase p22phox gene variants are associated with systemic oxidative stress biomarker responses to exercise training J Appl Physiol, November 1, 2005; 99(5): 1905 - 1911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Chess, M. A. O'Reilly, F. Sachs, and J. N. Finkelstein Reactive oxidant and p42/44 MAP kinase signaling is necessary for mechanical strain-induced proliferation in pulmonary epithelial cells J Appl Physiol, September 1, 2005; 99(3): 1226 - 1232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S R Hays, R E Ferrando, R Carter, H H Wong, and P G Woodruff Structural changes to airway smooth muscle in cystic fibrosis Thorax, March 1, 2005; 60(3): 226 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Djordjevic, R. S. BelAiba, S. Bonello, J. Pfeilschifter, J. Hess, and A. Gorlach Human Urotensin II Is a Novel Activator of NADPH Oxidase in Human Pulmonary Artery Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., March 1, 2005; 25(3): 519 - 525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Brar, Z. Corbin, T. P. Kennedy, R. Hemendinger, L. Thornton, B. Bommarius, R. S. Arnold, A. R. Whorton, A. B. Sturrock, T. P. Huecksteadt, et al. NOX5 NAD(P)H oxidase regulates growth and apoptosis in DU 145 prostate cancer cells Am J Physiol Cell Physiol, August 1, 2003; 285(2): C353 - C369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Parinandi, M. A. Kleinberg, P. V. Usatyuk, R. J. Cummings, A. Pennathur, A. J. Cardounel, J. L. Zweier, J. G. N. Garcia, and V. Natarajan Hyperoxia-induced NAD(P)H oxidase activation and regulation by MAP kinases in human lung endothelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L26 - L38. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
