| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print November 2, 2001
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00216.2001
Submitted on June 12, 2001
Accepted on October 15, 2001
1 Anesthesiology, University of Alabama at Birmingham, Birmingham, AL, USA
2 Medicine, University of Alabama at Birmingham, Birmingham, AL, USA
3 Pathobiology and Genomics, University of Alabama at Birmingham, Birmingham, AL, USA
4 Environmental Protection Agency, Research Triangle Park, NC, USA
* To whom correspondence should be addressed. E-mail: sadis.matalon{at}ccc.uab.edu.
We investigated putative mechanisms by which human surfactant protein A (SP-A) effects killing of Klebsiella pneumoniae by human alveolar macrophages (AMs), isolated from bronchoalveolar lavagates of patients with transplanted lungs. Co-incubation of AMs with human SP-A (25 µg/ml) and Klebsiella resulted in a 68% decrease in total CFUs by 120 min as compared to AMs infected with Klebsiella in the absence of SP-A and this SP-A-mediated effect was abolished by preincubation with NG-monomethyl-L-arginine. Incubation of transplant AMs with SP-A increased intracellular [Ca2+]i by 70% and nitrite and nitrate (NOx) production by 45% (from 0.24 ± 0.02 to 1.3 ± 0.21 nmol*106AMs-1*h-1). Preincubation with BAPTA/AM inhibited the increase in intracellular [Ca2+] and abrogated the SP-A mediated Klebsiella phagocytosis and killing. In contrast, incubation of AMs from normal volunteers with SP-A decreased both [Ca2+]i and NOx production and did not result in killing of Klebsiella. Significant killing of Klebsiella was also seen in a cell-free system by sustained production of peroxynitrite (>1 µM/min) at pH 5, but not at 7.4. These findings indicate that SP-A mediates pathogen killing by AMs from transplant lungs by stimulating phagocytosis and production of reactive oxygen-nitrogen intermediates.
This article has been cited by other articles:
|
|
 |
|
  K. B. Adler and S. Matalon Highlights of the August Issue Am. J. Respir. Cell Mol. Biol., August 1, 2009; 41(2): 125 - 126. [Full Text] [PDF]
|
|
|
|
|
|
K. B. Adler and S. Matalon Highlights of the July Issue Am. J. Respir. Cell Mol. Biol., July 1, 2009; 41(1): 1 - 2. [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Mizgerd and S. J. Skerrett Animal models of human pneumonia Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L387 - L398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Serezani, J. Chung, M. N. Ballinger, B. B. Moore, D. M. Aronoff, and M. Peters-Golden Prostaglandin E2 Suppresses Bacterial Killing in Alveolar Macrophages by Inhibiting NADPH Oxidase Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 562 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Song and S. Matalon Modulation of alveolar fluid clearance by reactive oxygen-nitrogen intermediates Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L855 - L858. [Full Text] [PDF]
|
|
|
|
|
|
N. G. Hall, Y. Liu, J. M. Hickman-Davis, G. C. Davis, C. Myles, E. J. Andrews, S. Matalon, and J. D. Lang Jr. Bactericidal Function of Alveolar Macrophages in Mechanically Ventilated Rabbits Am. J. Respir. Cell Mol. Biol., June 1, 2006; 34(6): 719 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. C. Serezani, D. M. Aronoff, S. Jancar, P. Mancuso, and M. Peters-Golden Leukotrienes enhance the bactericidal activity of alveolar macrophages against Klebsiella pneumoniae through the activation of NADPH oxidase Blood, August 1, 2005; 106(3): 1067 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Matalon and J. R. Wright Surfactant Proteins and Inflammation: The Yin and the Yang Am. J. Respir. Cell Mol. Biol., December 1, 2004; 31(6): 585 - 586. [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Deng, X. Zeng, M. Newstead, T. A. Moore, W. C. Tsai, V. J. Thannickal, and T. J. Standiford STAT4 Is a Critical Mediator of Early Innate Immune Responses against Pulmonary Klebsiella Infection J. Immunol., September 15, 2004; 173(6): 4075 - 4083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Chen, C. M. Fuller, T. R. Kleyman, and S. Matalon Mutations in the extracellular loop of {alpha}-rENaC alter sensitivity to amiloride and reactive species Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1202 - F1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Hickman-Davis, J. Gibbs-Erwin, J. R. Lindsey, and S. Matalon Role of Surfactant Protein-A in Nitric Oxide Production and Mycoplasma Killing in Congenic C57BL/6 Mice Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 319 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Matalon, K. M. Hardiman, L. Jain, D. C. Eaton, M. Kotlikoff, J. P. Eu, J. Sun, G. Meissner, and J. S. Stamler Regulation of ion channel structure and function by reactive oxygen-nitrogen species Am J Physiol Lung Cell Mol Physiol, December 1, 2003; 285(6): L1184 - L1189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. O'Reilly, J. M. Hickman-Davis, I. C. Davis, and S. Matalon Hyperoxia Impairs Antibacterial Function of Macrophages Through Effects on Actin Am. J. Respir. Cell Mol. Biol., April 1, 2003; 28(4): 443 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. Fang and A. Vazquez-Torres Nitric oxide production by human macrophages: there's NO doubt about it Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L941 - L943. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
