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1 The Second Department of Internal medicine, Nagasaki University, Nagasaki, Japan; Department of Molecular Microbiology and Immunology, Division of Molecular and Critical Microbiology, Nagasaki University Graduate School of Medical Sciences, Nagasaki, Japan
2 The Second Department of Internal medicine, Nagasaki University, Nagasaki, Japan
* To whom correspondence should be addressed. E-mail: kyana-ngs{at}umin.ac.jp.
Long-term treatment of macrolide antibiotics is considered an effective treatment for DPB. While hypersecretion is common feature of this disease and it is known that macrolide inhibit mucin production, the mechanism of the effect on mucin production is unclear. The aim of our study was to determine the production of muc5ac core protein, a major core protein of mucin in airway secretion, and the effect of clarithromycin treatment on such production in a mouse model mimicking diffuse panbronchiolitis (DPB). Alcian blue (AB)-periodic acid-Schiff(PAS)-positive cells were detected in the lungs of Pseudomonas aeruginosa infected mice. Western blots of these mice showed muc5ac glycoprotein at day 1 and increased progressively from day 4 to day 14 after inoculation of bacteria. Clarithromycin (10 mg/kg/day for 7 days) significantly reduced the muc5ac expression at both the mRNA and protein levels. To investigate the role of molecules upstream in muc5ac regulation, we examined the role of mitogen-activated protein kinase (MAPK). Extracellular signal-regulated kinase (ERK)1/2 phosphorylation increased in the infected lung and decreased after treatment. Our results suggest that overproduction of muc5ac plays an important role in the pathogenesis of DPB and that clinical improvement following macrolide therapy seems to involve, at least in part, Kaneko et al., Page 3 of 32 its inhibition of mucin overproduction, through modulation of intracellular signal transduction.
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