AJP - Lung Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (November 15, 2002). doi:10.1152/ajplung.00218.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/2/L376    most recent
00218.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thome, U.
Right arrow Articles by Matalon, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thome, U.
Right arrow Articles by Matalon, S.

Articles in PresS, published online ahead of print November 15, 2002
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00218.2002
Submitted on July 8, 2002
Accepted on October 18, 2002

Modulation of Sodium Transport in Fetal Alveolar Epithelial Cells by Oxygen and Corticosterone

Ulrich Thome1, Ian C. Davis2, Susie V. Nguyen2, Brent J. Shelton3, and Sadis Matalon4*

1 Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL, USA
2 Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL, USA
3 Department of Biostatistics, University of Alabama at Birmingham, Birmingham, AL, USA
4 Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL, USA; Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL, USA

* To whom correspondence should be addressed. E-mail: sadis{at}uab.edu.

Regulation of active Na+ transport across fetal distal lung epithelial cells (FDLE) by corticoster(CST), corticotropin-releasing hormone (CRH) and oxygen tension may be crucial for post-natal adaptation. FDLE isolated from 19 d rat fetuses (term: 22d) were grown on permeable supports to confluent monolayers (duration 3 d) in 2.5, 5, 12, or 20% O2 with 5% CO2, balance N2 and mounted in Ussing chambers for measurement of short circuit currents (Isc). FDLE monolayers grown in 20% O2 had significantly higher levels of total ISC, and their amiloride-sensitve (Iamil) and ouabain-sensitive (Iouab) components than hypoxic cells. Values (µA/cm2 ±SE) for 2.5-5% O2 and 20% O2 were, respectively: ISC 5.3±0.2 vs. 8.4±0.3 (p<0.001), Iamil 3.4±0.2 vs. 4.3±0.2 (p<0.01), Iouab 3.4±0.6 vs. 9.1±0.6 (p<0.001). Addition of CST but not CRH to the culture medium at any O2 concentration increased amiloride-sensitive currents. FDLE cells grown at 5% O2 expressed significantly lower levels of {alpha}, {beta} and {gamma}ENaC, and of the {alpha}1 Na,K-ATPase, as determined by Western blotting. We conclude that higher O2 concentrations increased total vectorial Na+ transport, as well as the function of Na,K-ATPase and apical amiloride-sensitive Na+ conductance, whereas CST only increased ENaC function.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
H.-G. Nie, L. Chen, D.-Y. Han, J. Li, W.-F. Song, S.-P. Wei, X.-H. Fang, X. Gu, S. Matalon, and H.-L. Ji
Regulation of epithelial sodium channels by cGMP/PKGII
J. Physiol., June 1, 2009; 587(11): 2663 - 2676.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H.-G. Nie, T. Tucker, X.-F. Su, T. Na, J.-B. Peng, P. R. Smith, S. Idell, and H.-L. Ji
Expression and Regulation of Epithelial Na+ Channels by Nucleotides in Pleural Mesothelial Cells
Am. J. Respir. Cell Mol. Biol., May 1, 2009; 40(5): 543 - 554.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. X. L. Zhang, J. J. Miller, D. B. Stolz, L. D. Serpero, W. Zhao, D. Gozal, and Y. Wang
Type I Epithelial Cells Are the Main Target of Whole-Body Hypoxic Preconditioning in the Lung
Am. J. Respir. Cell Mol. Biol., March 1, 2009; 40(3): 332 - 339.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
V. Bhalla and K. R. Hallows
Mechanisms of ENaC Regulation and Clinical Implications
J. Am. Soc. Nephrol., October 1, 2008; 19(10): 1845 - 1854.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Yu, H.-F. Bao, J. L. Self, D. C. Eaton, and M. N. Helms
Aldosterone-induced increases in superoxide production counters nitric oxide inhibition of epithelial Na channel activity in A6 distal nephron cells
Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1666 - F1677.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Bhalla, N. M. Oyster, A. C. Fitch, M. A. Wijngaarden, D. Neumann, U. Schlattner, D. Pearce, and K. R. Hallows
AMP-activated Kinase Inhibits the Epithelial Na+ Channel through Functional Regulation of the Ubiquitin Ligase Nedd4-2
J. Biol. Chem., September 8, 2006; 281(36): 26159 - 26169.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Otulakowski, B. Rafii, M. Harris, and H. O'Brodovich
Oxygen and Glucocorticoids Modulate {alpha}ENaC mRNA Translation in Fetal Distal Lung Epithelium
Am. J. Respir. Cell Mol. Biol., February 1, 2006; 34(2): 204 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. M. Hardiman, C. M. McNicholas-Bevensee, J. Fortenberry, C. T. Myles, B. Malik, D. C. Eaton, and S. Matalon
Regulation of Amiloride-Sensitive Na+ Transport by Basal Nitric Oxide
Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 720 - 728.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. A. Weisz and J. P. Johnson
Noncoordinate regulation of ENaC: paradigm lost?
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F833 - F842.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. L. Mandell, D. C. Devor, J. V. Madia, C.-Y. Lo, H. Hake, and P. A. Hebda
The effect of changes in ambient oxygen concentration on the bioelectric properties of middle ear mucosa
Am J Physiol Cell Physiol, September 1, 2003; 285(3): C618 - C622.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.