| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print November 15, 2002
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00218.2002
Submitted on July 8, 2002
Accepted on October 18, 2002
1 Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL, USA
2 Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL, USA
3 Department of Biostatistics, University of Alabama at Birmingham, Birmingham, AL, USA
4 Department of Anesthesiology, University of Alabama at Birmingham, Birmingham, AL, USA; Department of Pediatrics, University of Alabama at Birmingham, Birmingham, AL, USA
* To whom correspondence should be addressed. E-mail: sadis{at}uab.edu.
Regulation of active Na+ transport across fetal distal lung epithelial cells (FDLE) by corticoster(CST), corticotropin-releasing hormone (CRH) and oxygen tension may be crucial for post-natal adaptation. FDLE isolated from 19 d rat fetuses (term: 22d) were grown on permeable supports to confluent monolayers (duration 3 d) in 2.5, 5, 12, or 20% O2 with 5% CO2, balance N2 and mounted in Ussing chambers for measurement of short circuit currents (Isc). FDLE monolayers grown in 20% O2 had significantly higher levels of total ISC, and their amiloride-sensitve (Iamil) and ouabain-sensitive (Iouab) components than hypoxic cells. Values (µA/cm2 ±SE) for 2.5-5% O2 and 20% O2 were, respectively: ISC 5.3±0.2 vs. 8.4±0.3 (p<0.001), Iamil 3.4±0.2 vs. 4.3±0.2 (p<0.01), Iouab 3.4±0.6 vs. 9.1±0.6 (p<0.001). Addition of CST but not CRH to the culture medium at any O2 concentration increased amiloride-sensitive currents. FDLE cells grown at 5% O2 expressed significantly lower levels of 
, 
and 
ENaC, and of the 1 Na,K-ATPase, as determined by Western blotting. We conclude that higher O2 concentrations increased total vectorial Na+ transport, as well as the function of Na,K-ATPase and apical amiloride-sensitive Na+ conductance, whereas CST only increased ENaC function.
This article has been cited by other articles:
|
|
 |
|
  L. Yu, H.-F. Bao, J. L. Self, D. C. Eaton, and M. N. Helms Aldosterone-induced increases in superoxide production counters nitric oxide inhibition of epithelial Na channel activity in A6 distal nephron cells Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1666 - F1677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bhalla, N. M. Oyster, A. C. Fitch, M. A. Wijngaarden, D. Neumann, U. Schlattner, D. Pearce, and K. R. Hallows AMP-activated Kinase Inhibits the Epithelial Na+ Channel through Functional Regulation of the Ubiquitin Ligase Nedd4-2 J. Biol. Chem., September 8, 2006; 281(36): 26159 - 26169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Otulakowski, B. Rafii, M. Harris, and H. O'Brodovich Oxygen and Glucocorticoids Modulate {alpha}ENaC mRNA Translation in Fetal Distal Lung Epithelium Am. J. Respir. Cell Mol. Biol., February 1, 2006; 34(2): 204 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Hardiman, C. M. McNicholas-Bevensee, J. Fortenberry, C. T. Myles, B. Malik, D. C. Eaton, and S. Matalon Regulation of Amiloride-Sensitive Na+ Transport by Basal Nitric Oxide Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 720 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Weisz and J. P. Johnson Noncoordinate regulation of ENaC: paradigm lost? Am J Physiol Renal Physiol, November 1, 2003; 285(5): F833 - F842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Mandell, D. C. Devor, J. V. Madia, C.-Y. Lo, H. Hake, and P. A. Hebda The effect of changes in ambient oxygen concentration on the bioelectric properties of middle ear mucosa Am J Physiol Cell Physiol, September 1, 2003; 285(3): C618 - C622. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
