| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Physiology, New York Medical College, Valhalla, NY, USA
2 Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, USA
* To whom correspondence should be addressed. E-mail: mike_wolin{at}nymc.edu.
An ERK MAP kinase-mediated contractile mechanism previously reported to be activated by peroxide and stretch in bovine coronary arteries is shown in this study to be present in endothelium denuded bovine pulmonary arteries, and subject to regulation by modulation of cytosolic NAD(H) redox through the lactate dehydrogenase reaction. While our previous work identified an acute pO2-dependent peroxide-mediated relaxation of bovine pulmonary arteries on exposure to lactate, a 30 minute treatment with 10 mM lactate enhanced ERK phosphorylation and increased force generation to 30 mM KCl. Hypoxia inhibited these responses to lactate. Increases in ERK phosphorylation and the enhancement of force generation by lactate and stretch are attenuated in the presence of inhibitors of Nox oxidase (0.1 mM apocynin) or ERK activation (10 µM PD98059) and by 0.1 mM ebselen. Additionally, incubation of pulmonary arteries with 10 mM pyruvate lowered basal levels of ERK phosphorylation and it inhibited both the ERK phosphorylation and the enhancement in force generation to 30 mM KCl caused by stretch. Treatment of pulmonary arteries with the thiol oxidant diamide (1µM) elicited what appears to be a peroxide-independent increase in force and ERK phosphorylation that were both attenuated by PD98059. Thus, pulmonary arteries possess a peroxideelicited contractile mechanism involving ERK MAP kinase which is stimulated by stretch and regulated through the control of Nox oxidase activity by the availability of cytosolic NADH.
This article has been cited by other articles:
|
|
 |
|
  Q. Gao, X. Zhao, M. Ahmad, and M. S. Wolin Mitochondrial-derived hydrogen peroxide inhibits relaxation of bovine coronary arterial smooth muscle to hypoxia through stimulation of ERK MAP kinase Am J Physiol Heart Circ Physiol, December 1, 2009; 297(6): H2262 - H2269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Wolin Reactive oxygen species and the control of vascular function Am J Physiol Heart Circ Physiol, March 1, 2009; 296(3): H539 - H549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Bagi, N. Erdei, and A. Koller High intraluminal pressure via H2O2 upregulates arteriolar constrictions to angiotensin II by increasing the functional availability of AT1 receptors Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H835 - H841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schleicher, B. R. Shepherd, Y. Suarez, C. Fernandez-Hernando, J. Yu, Y. Pan, L. M. Acevedo, G. S. Shadel, and W. C. Sessa Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence J. Cell Biol., January 10, 2008; 180(1): 101 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ding, A. Chapman, R. Boyd, and H. D. Wang ERK activation contributes to regulation of spontaneous contractile tone via superoxide anion in isolated rat aorta of angiotensin II-induced hypertension Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2997 - H3005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ardanaz and P. J. Pagano Hydrogen peroxide as a paracrine vascular mediator: regulation and signaling leading to dysfunction. Experimental Biology and Medicine, March 1, 2006; 231(3): 237 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Mingone, S. A. Gupte, N. Ali, R. A. Oeckler, and M. S. Wolin Thiol oxidation inhibits nitric oxide-mediated pulmonary artery relaxation and guanylate cyclase stimulation Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L549 - L557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Wolin and S. A. Gupte Novel Roles for Nox Oxidases in Cardiac Arrhythmia and Oxidized Glutathione Export in Endothelial Function Circ. Res., September 30, 2005; 97(7): 612 - 614. [Full Text] [PDF]
|
|
|
|
|
|
M. S. Wolin, M. Ahmad, and S. A. Gupte Oxidant and redox signaling in vascular oxygen sensing mechanisms: basic concepts, current controversies, and potential importance of cytosolic NADPH Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L159 - L173. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
