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Articles in PresS, published online ahead of print October 12, 2001
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00227.2001
Submitted on June 18, 2001
Accepted on December 31, 1969
1 Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
2 Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA, USA
* To whom correspondence should be addressed. E-mail: batekenn{at}mail.med.upenn.edu.
A culture system designed to maintain the differentiated characteristics of rat type II cells based on protocols used for human fetal lung pneumocytes was investigated. Type II cells were isolated from either adult rats using elastase (adult type II cells) or young rats (4-11 days postnatal) using collagenase and trypsin (young type II cells) and were incubated with dexamethasone (Dex, 10 nM) and cAMP (0.1mM). By day 4 of culture with hormone treatment, the mRNA levels in adult type II cells were less than 3% of day 0 values while SP-A protein content was 26%. However, young type II cells maintained lamellar bodies and microvilli and secreted phospholipid in response to ATP. SP-A, B and C mRNA levels were elevated to 159, 350, and 39 %, respectively, of day 0 values with a synergistic response to Dex and cAMP, while SP-A protein content rose to 119%. Surfactant mRNA and protein did not recover in cells cultured without hormones. This cell culture system restored surfactant components in rat type II cells.
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