| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Environmental Health Sciences of Public Health, University of Alabama at Birmingham, Birmingham, AL, USA
2 Depatments of Medicine and Pathology, University of Alabama at Birmingham, Birmingham, AL, USA
3 Department of Anesthesiology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA
* To whom correspondence should be addressed. E-mail: rliu{at}uab.edu.
Transforming growth factor beta (TGF-
) is a potent fibrogenic cytokine. The molecular mechanism underlying TGF-'s fibrogenesis, however, has not been completely elucidated. In this study, we showed that TGF- decreased the intracellular GSH content in murine embryo fibroblasts (NIH3T3), which was followed by an increase in collagen I mRNA content and collagen protein production. Prevention of GSH depletion with N-acetylcysteine (NAC), GSH, or GSH ester abrogated TGF--stimulated collagen production while decreasing intracellular GSH content with L-buthionine S, R-sulfoximine (BSO), an inhibitor of de novo GSH synthesis, enhanced TGF--stimulated collagen production. These results suggest that GSH depletion induced by TGF- may mediate TGF--stimulated collagen production. In addition, we showed that TGF- stimulated superoxide production and increased release of hydrogen peroxide (H2O2) from the cells while GSH ester decreased the basal and TGF-/glucose oxidase stimulated H2O2 release. H2O2, exogenously added or continuously generated by glucose oxidase, enhanced TGF--stimulated collagen production while suppression of superoxide production by diphenyliodonium (DPI), a NAD(P)H oxidase inhibitor, blocked TGF--stimulated collagen production. These data further suggest that reactive oxygen species (ROS) are involved in TGF--stimulated collagen production and that the effect of GSH depletion on TGF--stimulated collagen production may be mediated by facilitating ROS signaling.
This article has been cited by other articles:
|
|
 |
|
  J. Behr and J. H. Ryu Pulmonary hypertension in interstitial lung disease Eur. Respir. J., June 1, 2008; 31(6): 1357 - 1367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Fu, S. Zheng, S. C. Lu, and A. Chen Epigallocatechin-3-gallate Inhibits Growth of Activated Hepatic Stellate Cells by Enhancing the Capacity of Glutathione Synthesis Mol. Pharmacol., May 1, 2008; 73(5): 1465 - 1473. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Muro, F. A. Moretti, B. B. Moore, M. Yan, R. G. Atrasz, C. A. Wilke, K. R. Flaherty, F. J. Martinez, J. L. Tsui, D. Sheppard, et al. An Essential Role for Fibronectin Extra Type III Domain A in Pulmonary Fibrosis Am. J. Respir. Crit. Care Med., March 15, 2008; 177(6): 638 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. K. Vayalil, K. E. Iles, J. Choi, A.-K. Yi, E. M. Postlethwait, and R.-M. Liu Glutathione suppresses TGF-beta-induced PAI-1 expression by inhibiting p38 and JNK MAPK and the binding of AP-1, SP-1, and Smad to the PAI-1 promoter Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1281 - L1292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lajoie-Kadoch, P. Joubert, S. Letuve, A. J. Halayko, J. G. Martin, A. Soussi-Gounni, and Q. Hamid TNF-{alpha} and IFN-{gamma} inversely modulate expression of the IL-17E receptor in airway smooth muscle cells Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1238 - L1246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Marian, V. Senthil, S. N. Chen, and R. Lombardi Antifibrotic Effects of Antioxidant N-Acetylcysteine in a Mouse Model of Human Hypertrophic Cardiomyopathy Mutation J. Am. Coll. Cardiol., February 21, 2006; 47(4): 827 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Vayalil, M. Olman, J. E. Murphy-Ullrich, E. M. Postlethwait, and R.-M. Liu Glutathione restores collagen degradation in TGF-{beta}-treated fibroblasts by blocking plasminogen activator inhibitor-1 expression and activating plasminogen Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L937 - L945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Demedts, J. Behr, R. Buhl, U. Costabel, R. Dekhuijzen, H. M. Jansen, W. MacNee, M. Thomeer, B. Wallaert, F. Laurent, et al. High-Dose Acetylcysteine in Idiopathic Pulmonary Fibrosis. N. Engl. J. Med., November 24, 2005; 353(21): 2229 - 2242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cheng, M. M. D. Encarnacion, G. M. Warner, C. E. Gray, K. A. Nath, and J. P. Grande TGF-{beta}1 stimulates monocyte chemoattractant protein-1 expression in mesangial cells through a phosphodiesterase isoenzyme 4-dependent process Am J Physiol Cell Physiol, October 1, 2005; 289(4): C959 - C970. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
