AJP - Lung Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (December 3, 2004). doi:10.1152/ajplung.00231.2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
288/4/L633    most recent
00231.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ashare, A.
Right arrow Articles by Hunninghake, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ashare, A.
Right arrow Articles by Hunninghake, G. W.
Submitted on June 22, 2004
Accepted on November 30, 2004

The anti-inflammatory response is associated with mortality and severity of infection in sepsis

Alix Ashare1*, Linda S. Powers1, Noah S. Butler1, Kevin C. Doerschug1, Martha M. Monick1, and Gary W. Hunninghake2

1 Department of Internal Medicine, Division of Pulmonary and Critical and Occupational Medicine, University of Iowa College of Medicine, Iowa City, Iowa, USA
2 Department of Internal Medicine, Division of Pulmonary and Critical and Occupational Medicine, University of Iowa College of Medicine, Iowa City, Iowa, USA; Internal Medicine, Veterans' Administration Medical Center, Iowa City, Iowa, USA

* To whom correspondence should be addressed. E-mail: alix-ashare{at}uiowa.edu.

Using a murine model of sepsis, we found that the balance of tissue pro- to anti-inflammatory cytokines directly correlated with severity of infection and mortality. Sepsis was induced in C57BL/6 mice by cecal ligation and puncture (CLP). Liver tissue was analyzed for levels of interleukin (IL)-1{beta}, IL-1 receptor antagonist (IL-1ra), tumor necrosis factor alpha (TNF{alpha}), and soluble TNF receptor 1 (sTNFR1) by ELISA. Bacterial DNA was measured using quantitative real-time PCR. After CLP, early predominance of pro-inflammatory cytokines (6 hours) transitioned to anti-inflammatory predominance at 24 hours. The elevated anti-inflammatory cytokines were mirrored by increased tissue bacterial levels. The degree of anti-inflammatory response compared to pro-inflammatory response correlated with the bacterial concentration. To modulate the timing of the anti-inflammatory response, mice were treated with IL-1ra prior to CLP. This resulted in decreased pro-inflammatory cytokines, earlier bacterial load and increased mortality. These studies show that the initial tissue pro-inflammatory response to sepsis is followed by an anti-inflammatory response. The anti-inflammatory phase is associated with increased bacterial load and mortality. These data suggest that it is the timing and magnitude of the anti-inflammatory response that predicts severity of infection in a murine model of sepsis.




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
A. A. Steiner, D. L. Oliveira, J. L. Roberts, S. R. Petersen, and A. A. Romanovsky
Nicotine administration and withdrawal affect survival in systemic inflammation models
J Appl Physiol, October 1, 2008; 105(4): 1028 - 1034.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. P. Miles
How do we solve the puzzle of unintended consequences of inflammation? Systematically
J Appl Physiol, October 1, 2008; 105(4): 1023 - 1025.
[Full Text] [PDF]

Home page
 FASEB J.Home page
M. A. Flierl, D. Rittirsch, B. A. Nadeau, D. E. Day, F. S. Zetoune, J. V. Sarma, M. S. Huber-Lang, and P. A. Ward
Functions of the complement components C3 and C5 during sepsis
FASEB J, October 1, 2008; 22(10): 3483 - 3490.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Ashare, A. B. Nymon, K. C. Doerschug, J. M. Morrison, M. M. Monick, and G. W. Hunninghake
Insulin-like Growth Factor-1 Improves Survival in Sepsis via Enhanced Hepatic Bacterial Clearance
Am. J. Respir. Crit. Care Med., July 15, 2008; 178(2): 149 - 157.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
X. Ye, J. Ding, X. Zhou, G. Chen, and S. F. Liu
Divergent roles of endothelial NF-{kappa}B in multiple organ injury and bacterial clearance in mouse models of sepsis
J. Exp. Med., June 9, 2008; 205(6): 1303 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Journals of Gerontology Series A: Biological Sciences and Medical SciencesHome page
M. Jylha, P. Paavilainen, T. Lehtimaki, S. Goebeler, P. J. Karhunen, A. Hervonen, and M. Hurme
Interleukin-1 Receptor Antagonist, Interleukin-6, and C-Reactive Protein as Predictors of Mortality in Nonagenarians: The Vitality 90+ Study
J. Gerontol. A Biol. Sci. Med. Sci., September 1, 2007; 62(9): 1016 - 1021.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Hegde, H. Zhang, S. M. Moochhala, and M. Bhatia
Neurokinin-1 receptor antagonist treatment protects mice against lung injury in polymicrobial sepsis
J. Leukoc. Biol., September 1, 2007; 82(3): 678 - 685.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Ashare, M. M. Monick, L. S. Powers, T. Yarovinsky, and G. W. Hunninghake
Severe Bacteremia Results in a Loss of Hepatic Bacterial Clearance
Am. J. Respir. Crit. Care Med., March 15, 2006; 173(6): 644 - 652.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.