AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (August 9, 2002). doi:10.1152/ajplung.00268.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/6/L1220    most recent
00268.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pandya, H. C.
Right arrow Articles by Hirst, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pandya, H. C.
Right arrow Articles by Hirst, S. J.

Articles in PresS, published online ahead of print August 9, 2002
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00268.2001
Submitted on July 18, 2001
Accepted on August 2, 2002

Oxygen regulates mitogen-stimulated proliferation of fetal human airway smooth muscle cells

Hitesh C. Pandya1, Vladimir A. Snetkov1, Charles H. C. Twort1, Jeremy P. T. Ward1, and Stuart J. Hirst1*

1 Department of Respiratory Medicine and Allergy, The Guy's, King's and St. Thomas' School of Medicine, King's College London, London, United Kingdom

* To whom correspondence should be addressed. E-mail: stuart.hirst{at}kcl.ac.uk.

Increased airway smooth muscle (ASM) content is characteristic of infants with chronic lung disease of prematurity/bronchopulmonary dysplasia (CLD-BPD). Oxygen therapy, reactive oxygen species (ROS) and immature antioxidant defences are major risk factors in CLD-BPD, but their interrelationship is unclear. The direct effects of raised PO2 and modulation of ROS were examined on proliferation of cultured fetal human ASM cells. A bell-shaped relationship was found between PO2 and DNA synthesis induced by FBS, PDGF and bFGF, with peak responses occurring at 10kPa PO2. Changes in DNA synthesis by PO2 did not occur in the absence of mitogen. ROS generation, estimated by dichlorodihydrofluorescein oxidation, was increased by mitogens, but was unaffected by non-mitogens (bradykinin, histamine). There was an inverse relationship between ROS generation and PO2, and mitogen-induced ROS generation was substantially potentiated as the PO2 fell. H2O2 mimicked the effect of PO2 on FBS-stimulated proliferation, whereas treatment with antioxidants (GSH, N-acetylcysteine) reduced it. These data demonstrate that increases in PO2 above levels found in utero modulate proliferation of fetal ASM cells, but only in the presence of growth factors. They also strongly suggest that under these conditions proliferation is mediated in part by generation of ROS.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Sturrock, T. P. Huecksteadt, K. Norman, K. Sanders, T. M. Murphy, P. Chitano, K. Wilson, J. R. Hoidal, and T. P. Kennedy
Nox4 mediates TGF-beta1-induced retinoblastoma protein phosphorylation, proliferation, and hypertrophy in human airway smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1543 - L1555.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Fantozzi, O. Platoshyn, A. H. Wong, S. Zhang, C. V. Remillard, M. R. Furtado, O. V. Petrauskene, and J. X.-J. Yuan
Bone morphogenetic protein-2 upregulates expression and function of voltage-gated K+ channels in human pulmonary artery smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L993 - L1004.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. Cogo, G. Napolitano, M. C. Michoud, D. R. Barbon, M. Ward, and J. G. Martin
Effects of hypoxia on rat airway smooth muscle cell proliferation
J Appl Physiol, April 1, 2003; 94(4): 1403 - 1409.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.