| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Otorhinolaryngology, Yonsei University Collge of Medicine, Seoul, Kuwait; The Airway Mucus Institue, Yonsei University College of Medicine, Seoul, Korea, Republic of
2 Department of Otorhinolaryngology, Yonsei University Collge of Medicine, Seoul, Kuwait
3 The Airway Mucus Institue, Yonsei University College of Medicine, Seoul, Korea, Republic of
4 Department of Pharmacology, Yonsei University College of Medicine, Seoul, Korea, Republic of
5 Department of Otorhinolaryngology, Yonsei University Collge of Medicine, Seoul, Kuwait; Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea, Republic of
6 Department of Otorhinolaryngology, Yonsei University Collge of Medicine, Seoul, Kuwait; The Airway Mucus Institue, Yonsei University College of Medicine, Seoul, Korea, Republic of; Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea, Republic of
* To whom correspondence should be addressed. E-mail: jhyoon{at}yumc.yonsei.ac.kr.
Extracellular purines and pyrimidines regulate various physiological responses via the cell surface receptors known as purinoreceptors, and may exert autocrine or paracrine effects on ion transport, fluid transport, the ciliary beat frequency and mucin secretion. Therefore, this study aims to investigat the expression patterns of the purinoreceptors in normal human nasal epithelial (NHNE) cells. In RT-PCR, the mRNAs for several P2X (P2X3, P2X4, P2X7) and P2Y (P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring [Ca2+]i increases in membrane-specific manner using a double-perfusion chamber. Absence of the responses of -Me ATP and 2MeS-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase was concerned. Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes express P2Y2 receptor. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by the immunoblotting using specific antibodies. In addition, the results with BzATP indicated that the P2Y11 receptor may be present on the luminal side. In conclusion, the NHNE cells express functionally active P2Y2, P2Y6 and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells.
This article has been cited by other articles:
|
|
 |
|
  S. S.P. Wildman, J. Marks, C. M. Turner, L. Yew-Booth, C. M. Peppiatt-Wildman, B. F. King, D. G. Shirley, W. Wang, and R. J. Unwin Sodium-Dependent Regulation of Renal Amiloride-Sensitive Currents by Apical P2 Receptors J. Am. Soc. Nephrol., April 1, 2008; 19(4): 731 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Burnstock Physiology and Pathophysiology of Purinergic Neurotransmission Physiol Rev, April 1, 2007; 87(2): 659 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Shaykhiev, C. Beisswenger, K. Kandler, J. Senske, A. Puchner, T. Damm, J. Behr, and R. Bals Human endogenous antibiotic LL-37 stimulates airway epithelial cell proliferation and wound closure Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L842 - L848. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
