AJP - Lung Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Lung Cell Mol Physiol (March 22, 2002). doi:10.1152/ajplung.00302.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/2/L256    most recent
00302.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rice, W. R.
Right arrow Articles by Weaver, T. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Rice, W. R.
Right arrow Articles by Weaver, T. E.

Articles in PresS, published online ahead of print March 22, 2002
Am J Physiol Lung Cell Mol Physiol, 10.1152/ajplung.00302.2001
Submitted on August 2, 2001
Accepted on March 15, 2002

Maintenance of Mouse Type II Cell Phenotype in vitro

Ward R. Rice1*, Juliana J. Conkright1, Cheng-Lun Na1, Machiko Ikegami1, John M. Shannon1, and Timothy E. Weaver1

1 Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, OH, USA

* To whom correspondence should be addressed. E-mail: ricew0{at}chmcc.org.

The purpose of this study was to identify culture conditions for maintenance of isolated mouse type II cells with intact surfactant protein and phospholipid production. Type II cells were isolated from 6 week-old mice and cultured on Matrigel matrix:rat tail collagen (70:30, v:v) in bronchial epithelial cell growth medium (BEGM) minus hydrocortisone plus 5% charcoal-stripped fetal bovine serum and 10 ng/ml KGF. Under these conditions, type II cells actively produced surfactant phospholipids and proteins for at least 7 days. Synthesis and secretion of surfactant phospholipids and surfactant proteins A, B, C, and D declined on day 1 of culture, but recovered by day 3 reaching levels comparable to or exceeding freshly isolated cells by day 5. Abundant lamellar bodies were readily apparent in cells examined on days 1, 5, and 7 and a surfactant pellet was recovered by centrifugation of media harvested on each day of culture. Secretion of SP-B, SP-C and phosphatidylcholine was stimulated by the phorbol ester TPA and inhibited by Compound 48/80. When tested with a bubble surfactometer, surfactant secreted by type II cells on day 5 of culture lowered surface tension to 5.2±2.3 mN/m. This is the first description of the synthesis and secretion of a functional surfactant complex by mouse type II cells after 7 days in primary culture.




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. G. Lee, D. Hartl, H. Matsuura, F. M. Dunlop, P. D. Scotney, L. J. Fabri, A. D. Nash, N.-Y. Chen, C.-Y. Tang, Q. Chen, et al.
Endogenous IL-11 Signaling Is Essential in Th2- and IL-13-Induced Inflammation and Mucus Production
Am. J. Respir. Cell Mol. Biol., December 1, 2008; 39(6): 739 - 746.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Prevention ResearchHome page
R. Nemenoff, A. M. Meyer, T. M. Hudish, A. B. Mozer, A. Snee, S. Narumiya, R. S. Stearman, R. A. Winn, M. Weiser-Evans, M. W. Geraci, et al.
Prostacyclin Prevents Murine Lung Cancer Independent of the Membrane Receptor by Activation of Peroxisomal Proliferator-Activated Receptor {gamma}
Cancer Prevention Research, October 1, 2008; 1(5): 349 - 356.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Ikegami, A. Falcone, and J. A. Whitsett
STAT-3 regulates surfactant phospholipid homeostasis in normal lung and during endotoxin-mediated lung injury
J Appl Physiol, June 1, 2008; 104(6): 1753 - 1760.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Yamasaki, H.-R. Kang, R. J. Homer, S. P. Chapoval, S. J. Cho, B. J. Lee, J. A. Elias, and C. G. Lee
P21 Regulates TGF-{beta}1-Induced Pulmonary Responses via a TNF-{alpha}-Signaling Pathway
Am. J. Respir. Cell Mol. Biol., March 1, 2008; 38(3): 346 - 353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. K. Sharma, L. G. Fernandez, A. S. Awad, I. L. Kron, and V. E. Laubach
Proinflammatory response of alveolar epithelial cells is enhanced by alveolar macrophage-produced TNF-{alpha} during pulmonary ischemia-reperfusion injury
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L105 - L113.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Wang, F. Gigliotti, S. P. Bhagwat, S. B. Maggirwar, and T. W. Wright
Pneumocystis stimulates MCP-1 production by alveolar epithelial cells through a JNK-dependent mechanism
Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1495 - L1505.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Deimling, K. Thompson, I. Tseu, J. Wang, R. Keijzer, A. K. Tanswell, and M. Post
Mesenchymal maintenance of distal epithelial cell phenotype during late fetal lung development
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L725 - L741.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. F. Barker, N. D. Manzo, K. L. Cotich, R. K. Shone, and A. B. Waxman
DNA Damage Induced by Hyperoxia: Quantitation and Correlation with Lung Injury
Am. J. Respir. Cell Mol. Biol., September 1, 2006; 35(3): 277 - 288.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Chen, B. A. Hyatt, M. L. Mucenski, R. J. Mason, and J. M. Shannon
Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells
PNAS, August 1, 2006; 103(31): 11724 - 11729.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Lin, A.-K. T. Perl, and J. M. Shannon
Erm/Thyroid Transcription Factor 1 Interactions Modulate Surfactant Protein C Transcription
J. Biol. Chem., June 16, 2006; 281(24): 16716 - 16726.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Lee, R. Reddy, L. Barsky, K. Weinberg, and B. Driscoll
Contribution of proliferation and DNA damage repair to alveolar epithelial type 2 cell recovery from hyperoxia
Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L685 - L694.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. E. De Paepe, Q. Mao, Y. Chao, J. L. Powell, L. P. Rubin, and S. Sharma
Hyperoxia-induced apoptosis and Fas/FasL expression in lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L647 - L659.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, T. D. Le Cras, W. D. Hardie, M. T. Stahlman, J. A. Whitsett, and T. R. Korfhagen
TGF-{alpha} perturbs surfactant homeostasis in vivo
Am J Physiol Lung Cell Mol Physiol, July 1, 2005; 289(1): L34 - L43.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Planes, C. Leyvraz, T. Uchida, M. A. Angelova, G. Vuagniaux, E. Hummler, M. Matthay, C. Clerici, and B. Rossier
In vitro and in vivo regulation of transepithelial lung alveolar sodium transport by serine proteases
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1099 - L1109.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. L. Nesslein, K. R. Melton, M. Ikegami, C.-L. Na, S. E. Wert, W. R. Rice, J. A. Whitsett, and T. E. Weaver
Partial SP-B deficiency perturbs lung function and causes air space abnormalities
Am J Physiol Lung Cell Mol Physiol, June 1, 2005; 288(6): L1154 - L1161.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, C.-L. Na, T. R. Korfhagen, and J. A. Whitsett
Surfactant protein D influences surfactant ultrastructure and uptake by alveolar type II cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2005; 288(3): L552 - L561.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
S. A. Blaine, A. M. Meyer, G. Hurteau, M. Wick, J. A. Hankin, R. C. Murphy, A. J. Dannenberg, M. W. Geraci, K. Subbaramaiah, and R. A. Nemenoff
Targeted over-expression of mPGES-1 and elevated PGE2 production is not sufficient for lung tumorigenesis in mice
Carcinogenesis, January 1, 2005; 26(1): 209 - 217.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. L. Miakotina, M. Agassandian, L. Shi, D. C. Look, and R. K. Mallampalli
Adenovirus stimulates choline efflux by increasing expression of organic cation transporter-2
Am J Physiol Lung Cell Mol Physiol, January 1, 2005; 288(1): L93 - L102.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. E. De Paepe, Q. Mao, M. Embree-Ku, L. P. Rubin, and F. I. Luks
Fas/FasL-mediated apoptosis in perinatal murine lungs
Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L730 - L742.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. L. Keith, Y. E. Miller, T. M. Hudish, C. E. Girod, S. Sotto-Santiago, W. A. Franklin, R. A. Nemenoff, T. H. March, S. P. Nana-Sinkam, and M. W. Geraci
Pulmonary Prostacyclin Synthase Overexpression Chemoprevents Tobacco Smoke Lung Carcinogenesis in Mice
Cancer Res., August 15, 2004; 64(16): 5897 - 5904.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
C. G. Lee, S. J. Cho, M. J. Kang, S. P. Chapoval, P. J. Lee, P. W. Noble, T. Yehualaeshet, B. Lu, R. A. Flavell, J. Milbrandt, et al.
Early Growth Response Gene 1-mediated Apoptosis Is Essential for Transforming Growth Factor {beta}1-induced Pulmonary Fibrosis
J. Exp. Med., August 2, 2004; 200(3): 377 - 389.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. I. Gobran and S. A. Rooney
Pulmonary surfactant secretion in briefly cultured mouse type II cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2004; 286(2): L331 - L336.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. R. Melton, L. L. Nesslein, M. Ikegami, J. W. Tichelaar, J. C. Clark, J. A. Whitsett, and T. E. Weaver
SP-B deficiency causes respiratory failure in adult mice
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L543 - L549.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. M. Roper, R. J. Staversky, J. N. Finkelstein, P. C. Keng, and M. A. O'Reilly
Identification and isolation of mouse type II cells on the basis of intrinsic expression of enhanced green fluorescent protein
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L691 - L700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Yang, A. Naltner, A. Kreiner, D. Yan, A. Cowen, H. Du, and C. Yan
An enhancer region determines hSP-B gene expression in bronchiolar and ATII epithelial cells in transgenic mice
Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L481 - L488.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.