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Am J Physiol Lung Cell Mol Physiol (January 23, 2004). doi:10.1152/ajplung.00304.2003
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Submitted on September 3, 2003
Accepted on January 16, 2004

A PROTEOMIC ANALYSIS OF PULMONARY EDEMA FLUID AND PLASMA IN PATIENTS WITH ACUTE LUNG INJURY

Russell P. Bowler1*, Beth Duda1, Edward D. Chan1, Jan J. Enghild2, Lorraine B. Ware3, Michael A. Matthay4, and Mark W. Duncan5

1 Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado, USA
2 Department of Molecular and Structural Biology, Aarhus University, Aarhus, Denmark
3 Department of Medicine, Vanderbilt University, Nashville, Tenn, USA
4 Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of California, San Francisco, California, USA
5 Biochemical Mass Spectrometry Facility, University of Colorado Health Sciences Center, Denver, Colorado, USA

* To whom correspondence should be addressed. E-mail: BowlerR{at}njc.org.

Proteomics is the large-scale analysis of protein profiles. This approach has not yet been reported in the study of acute lung injury (ALI). This study details protein profiles in plasma and pulmonary edema fluid (EF) from 16 ALI patients and plasma and bronchoalveolar lavage fluid (BALF) from 12 normal subjects. More than 300 distinct protein spots were evident in the EF and BALF of both normal subjects and ALI patients. Of these 158 were identified by matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In the plasma and EF protein profile of ALI patients there were multiple qualitative changes. For instance, in all normal subjects, but in only one of the ALI patients, seven distinct surfactant protein A isoforms were evident. Nearly all ALI patients also had protein spots that indicate truncation or other post-translational modifications. Several of these novel changes could serve as new biomarkers of lung injury.




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