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1 Department Of Pharmacology and Toxicology, East Carolina University, Brody School Of Medicine, Greenville, NC, USA
* To whom correspondence should be addressed. E-mail: mustafas{at}mail.ecu.edu.
We recently reported that adenosine caused bronchoconstriction and enhanced airway inflammation in an allergic mouse model. In this study, we further report the characterization of the subtype of adenosine receptor(s) involved in bronchoconstriction. NECA, a non-selective adenosine agonist, elicited bronchoconstriction in a dose-dependent manner. Little effects of CPA (A1-selective agonist) and CGS21680 (A2A-selective agonist) compared to NECA were observed in this model. CL-IB-MECA, an A3-selective receptor agonist, produced a dose-dependent bronchoconstrictor response, which was blocked by selective A3 antagonist, MRS1523. However, MRS1523 only partially inhibited NECA-induced bronchconstriction. Neither selective A1 nor A2A antagonists affected NECA-induced bronchconstriction. Enprofylline, a relatively selective A2B receptor antagonist, blocked partly NECA-induced bronchoconstriction. Furthermore, a combination of enprofylline and MRS1523 completely abolished NECA-induced bronchoconstrictor response. Using RT-PCR, we found that all four adenosine receptor subtypes are expressed in control lungs. Allergen sensitization and challenge significantly increased transcript levels of the A2B and A3 receptors whereas the A1 receptor message decreased. No change in transcript levels of A2A receptors was observed after allergen sensitization and challenge. These findings suggested that A2B and A3 adenosine receptors play an important role in adenosine-induced bronchoconstriction in our allergic mouse model. Finally, whether the airway effects of the receptor agonists/antagonists are direct or indirect needs further investigations.
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